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基因芯片-生物黑客兴起 修改基因手臂内置芯片可开门

发布时间：2017-08-12 所属栏目：生物黑客

一 : 生物黑客兴起 修改基因手臂内置芯片可开门

　　【61阅读IT新闻频道】一提到黑客，我们总能想到一个坐在电脑前，噼里啪啦敲着代码的人，他们偷偷侵入别人的设备，盗取他人的信息，或者是破坏他人的设备等。如今，我们或许要对这一印象做出改变。

　　据外媒报道，生物黑客是一刚起步的职业，是指那些修改动物、植物甚至人类的基因，以达到赋予这些组织新用途的专业人士。从功能上说，生物黑客其实已经取得了一些成果。相信随着时间的推移，它终将成为一种超越人们普遍认识的职业。

生物黑客兴起 修改基因手臂内置芯片可开门

　　报道称，在美国等一些世界大国，生物黑客已经走向成功。在美国西雅图创建了自己的生物黑客公司的阿马尔•格拉夫斯特拉认为，生物黑客就是把身体看作是一部能够适应各种试验和通过大脑去探索世界的简单机器的人。在生物黑客看来，身体是伟大的，但也是可以改变的。

　　格拉夫斯特拉认为，生物黑客是可以掀起一场革命的人，“因为人们正在回归到研究自己的道路上”。作为一名生物黑客，格拉夫斯特拉的研究在过去5到10年已经取得了相当大的进展。他指出，在美国已经形成了一个足够强大的生物黑客群体。

　　格拉夫斯特拉本人的情况就十分特殊，他在2005年时给自己的双手分别植入了无线射频芯片。借助这些芯片，他可以轻松地完成很多事情，比如开启电脑、打开房门或者启动汽车等。很多人开始对这些芯片产生兴趣。于是，格拉夫斯特拉创办了自己的名为“危险事物”的公司，主要任务就是致力于改善医疗界对移植的认识，并研发新的可植入设备。

　　报道称，他的公司由多位生物黑客组成。但是在西班牙，生物黑客作为一种职业尚未出现企业或实验室的形式，部分原因是缺少专家型人才和对这一职业的认识不足。格拉夫斯特拉认为，“企业和普通的科学家通常都会比较谨慎。生物黑客则更愿意承担一定风险，以证明理论的正确性”。

　　“人们应当开始重视某些机械装置的存在，比如近场通讯芯片(NFC)。它可以让人们在不需要钥匙或密码的情况下控制自己的手机和房门。”格拉夫斯特拉说。由他开展的首个项目之一就是在手臂上植入芯片，用来操控各种采用近场通讯技术的电子设备，比如智能手机等。

　　无论是在美国，还是在其他尚未在这一领域起步的国家，生物黑客已经成为一种现实，几年后它将彻底进入我们的日常生活。到那时，可以用手臂内的微芯片开启房门，或者用细菌做成的圆珠笔写字，人们将有太多的事情不得不从头开始适应。

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二 : 基因芯片分析

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三 : 51基因芯片

肺炎基因芯片结果生物信息学分析

基因芯片(genechip)（又称DNA芯片、生物芯片），最早在80年代中期提出，是指将许多特定的寡核苷酸片段或基因片段作为探针，有规律地排列固定于2cm x 2cm 的硅片、玻片等支持物，然后与待测的荧光标记样品的基因按碱基互补配对原理进行杂交，再通过激光共聚焦荧光检测系统等对芯片进行扫描，并配以计算机系统对每一探针上的荧光信号强度作出比较和检测，进而获取样品分子的数量和序列信息1-5。基因芯片技术也在1998年被列为年度自然科学领域十大进展之一，目前在实际应用方面，已广泛应用于疾病诊断和治疗、药物筛选、农作物的优育优选、司法鉴定、食品卫生监督、环境检测、国防、航天等许多领域。 生物信息学（bioinformatics）是利用应用数学、信息学、统计学和计算机科学的方法研究生物学的问题。目前的生物信息学基本上只是分子生物学与信息技术（尤其是互联网技术）的结合体。生物信息学的研究材料和结果就是各种各样的生物学数据，其研究工具是计算机，研究方法包括对生物学数据的搜索（收集和筛选）、处理（编辑、整理、管理和显示）及利用（计算、模拟）。目前主要的研究方向有：序列比对、基因识别、基因重组、蛋白质结构预测、基因表达、蛋白质反应的预测，以及建立进化模型。生物学技术往往生成大量的嘈杂数据，与数据挖掘类似，生物信息学利用数学工具从大量数据中提取有用的生物学信息。生物信息学所要处理的典型问题包括：重新组装在霰弹枪定序法测序过程中被打散的DNA序列，从蛋白质的氨基酸序列预测蛋白质结构，利用mRNA微阵列或质谱仪的数据检验基因调控的假说。

本节主要介绍利用生物信息学来分析和处理肺炎基因芯片结果的方法。

第一节 实验所需试剂与仪器

1．RNA样品制备试剂与仪器 TRIzol?试剂（Invitrogen life technologies），Biopulverizer 组织研磨器（biospec），Mini-Bead-Beater-16 研磨珠均质器（biospec）

2. 总RNA纯化及质检试剂和仪器 RNeasy小型试剂盒(Qiagen p/n 74904) ( Buffer RPE缓冲液RPE，Buffer RLT缓冲液RLT，RNeasy小型离心柱)，NanoDrop ND-1000，Baseline-ZERO DNA酶（EPICENTRE, Cat. No. DB0711K）

3. 标记反应试剂盒 快速Amp标记试剂盒，单标（Agilent p/n 5190-0442）

4. 标记/扩增的RNA的纯化和标记cDNA的质量控制试剂盒与仪器 RNeasy Mini Kit (Qiagen p/n 74104)，NanoDrop ND-1000

5. 杂交试剂盒与仪器 Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242):10X Blocking Agent, 10X封闭试剂, 25X Fragmentation Buffer, 25X片段化缓冲液, 2x GEx Hybridization Buffer HI-RPM, 2x GEx杂交缓冲液HI-RPM, 杂交室，不锈钢(Agilent p/n G2534A)。杂交室密封垫片基(Agilent p/n G2534-60003) , 杂交炉(Agilent p/n G2545A) ,安捷伦微阵列杂交室用杂交炉旋转器(Agilent p/n G2530-60029)

6. 芯片洗涤试剂盒与仪器 Gene Expression Wash Buffer 1 (Agilent p/n 5188-5325), Gene Expression Wash Buffer 2 (Agilent p/n 5188-5326), Magnetic stir bar (Corning p/n 401435), Magnetic stir plate (Corning p/n 6795-410), Slide-staining dish, with slide rack (Thermo Shandon p/n 121)

7. 扫描仪器 Agilent Microarray Scanner (Agilent p/n G2565BA)

8. Agilent Feature Extraction Software提取数据软件 Agilent Feature Extraction

第二节 实验步骤

一、RNA样品制备

样品分组：1-3正常肺组织(N)，4-6肺炎肺组织(F)

步骤：

1. 匀浆

a. 组织

a) Biopulverizer冰冻粉碎组织。

?

?

? 将BioPulverizer?置入浅盘并使用液氮充分冷却。 将预冷的组织放入研钵的孔内。 将研钵从浅容器中取出，放置在实验台，用锤子将研杵用力敲一到两下以磨碎组织（59012MS型）。

? 将粉碎物清出以用于进一步的匀浆或提取步骤。

b) Mini-Bead-Beater-16匀浆

? 在2ml螺盖微量离心管中将1.0mm微珠装至一半，然后加入TRIzol和组织，注意要将微量离

心管装满。*尽量排除管内的空气。*在匀浆过程中要使用带O形圈的螺盖微量离心管以免产

生气雾。*确保在旋上螺盖时口上无微珠。

? 将1到16个微量离心管放在架子上。*如需离心，则需要将它们对称排列。*拧上黑色塑料盖

子直至接触到微量离心管管盖顶部。*用手紧固盖子以固定好瓶子。不要过紧。

? 依样品类型定时。启动机器。

b. 贴壁细胞

直接在3.5cm直径培养皿里加入1ml TRIzol试剂以裂解细胞，并用移液管吹吸细胞裂解液几次。TRIzol试剂的加入量由培养皿的面积（1ml每平方厘米）而非细胞的量决定。

c. 悬浮细胞

细胞离心的沉淀加入TRIzol试剂并用移液管反复吹吸以裂解细胞。每5-10 × 10动物植物或酵母细胞或每1 × 10细菌细胞使用1ml的TRIzol试剂。在TRIzol试剂加入前不要洗涤细胞以免增加mRNA降解的可能。破碎一些酵母和细菌的细胞也可能需要匀浆。

2. 两相分离

已加TRIzol的样品于15到30°C放置5分钟，以便核酸蛋白复合体完全解离。每1 ml的TRIzol试剂匀浆的样品中加入0.2 ml的氯仿，盖紧管盖。手动剧烈振荡管体15秒后，15到30°C孵育2到3分钟。4°C下12,000 ×g离心15分钟。离心后混合液体将分为下层的红色酚氯仿相，中间层核上层的无色的水相。RNA全部被分配于水相中。水相的体积大约使匀浆时加入的TRIzol试剂的60%。

3. RNA沉淀

将水相转移到新离心管中。水相与异丙醇混合以沉淀其中的RNA，加入异丙醇的量为每个样品匀浆时加入1 ml TRIZOL试剂的此时加0.5 ml的异丙醇。混匀后15到30°C孵育10分钟后，于4°C 12,000 ×g离心10分钟。此时离心前不可见的RNA沉淀将在管底部和侧壁上形成胶状沉淀块。

4. RNA清洗

移去上清液，每1 ml TRIzol试剂匀浆的样品中加入至少1 ml的75%乙醇，清洗RNA沉淀。振荡后，4°

C 7,500 ×g离心5分钟。

5. 重新溶解RNA沉淀

最后，空气中干燥RNA沉淀5-10分钟，切勿真空离心干燥。注意RNA沉淀不要完全干燥，否则将大大降低RNA的可溶性。部分溶解的RNA样品A260 / 280比值将小于1.6。溶解RNA时，先加入无RNA酶的水用枪反复吹打几次，然后55到60°C孵育10分钟。获得的RNA溶液保存于- 70°C。

二、总RNA纯化及质检

操作前注意事项：

1. RPE缓冲液是以浓缩液的形式提供的，第一次使用前，按瓶上所述加入4倍体积的96-100%乙醇配成

工作溶液。

步骤：

1. 将以下组分在微量离心管中混合：

重新溶解的RNA

10×反应缓冲液 ≤85ul 10ul 76

Baseline-ZERO DNA酶

无RNA酶的水

总体积 2.

3.

4.

5. 37°C孵育30分钟。 加入350 μl 缓冲液RLT，混匀。 5ul X ul 100ul 加入250 μl 乙醇 (96–100%)，吸打混匀。无需离心，立即转入步骤5。 把700 微升的样品加入到安装在2毫升收集管上的RNeasy小型离心柱中，小心地盖上盖子，≥8000 x

g 离心15s，弃去流体。

6. 在RNeasy小柱中加入500 μl缓冲液RPE，小心地盖上盖子，≥8000 x g离心15s，洗涤RNeasy膜，

弃去流体。

7.

8.

9. 再次加入500 μl缓冲液RPE，盖上盖子，≥8000 x g离心2min洗涤RNeasy膜。 把RNeasy柱放在一新的2ml的收集管中，用过滤法去除旧的收集管，全速离心1分钟。 RNeasy柱转移到一新的1.5毫升的离心管中，吸取适量无RNA酶的水（请参考“RNA-QC”报告）直

接加入到RNeasy膜中。盖上管盖，≥8000 x g离心1分钟、洗脱。

10. RNA定量和质量控制（请参考“RNA-QC”报告）

三、标记反应

操作步骤：

1.

2.

3.

4.

5.

6. 将1μg总RNA加入到一支1.5ml的微量离心管中。 加入1.2μl的T7 Promoter引物 加无核酸酶的水至总反应体积11.5μl 将反应体系置65°C循环水浴10分钟使引物和模板变性。 将反应体系置冰上并静置5分钟。 将下表所列组分按顺序加入并轻轻混合以配置成cDNA Master Mix，置冰上，现配现用。

5×First Strand Buffer

0.1M DTT

10mM dNTP mix

MMLV-RT

RNaseOut

Total volume 7.

8.

9. Volume(ul) per reaction 4 2 1 1 0.5 8.5 将每样品管在微量离心机中简单甩一下，使管壁和管盖上的溶液离心下来。将离心管放回冰上。 在每样品管中加入8.5μl的cDNA Master Mix并上下吹吸混合。 将样品于40°C循环水浴中温育2小时。

10. 将样品转至65°C循环水浴中温育15分钟。

11. 将样品转至冰上并静置5分钟。

12. 将每样品管在微量离心机中简单甩一下，使管壁和管盖上的溶液离心下来。

13. 将下表所列组分按顺序加入并在室温轻轻吹吸混合以配置成Transcription Master Mix，现配现用。

Nuclease-free water

4X Transcription Buffer Volume(ul) per reaction 15.3 20

0.1 M DTT

NTP mix

50% PEG

RNaseOUT

Inorganic pyrophosphatase

T7 RNA Polymerase

Cyanine-3-CTP

Total volume 6 8 6.4 0.5 0.6 0.8 2.4 60

14. 在每样品管中加入60μl Transcription Master Mix。轻轻吹吸混匀。

15. 将样品于40°C循环水浴中温育2小时。

四、标记/扩增的RNA的纯化和标记cDNA的质量控制

操作步骤：

1.

2.

3.

4. 加入20μl无核酸酶的水至cRNA样品中至总体积100μl 加入350μl的RLT缓冲液并吹吸充分混匀 加入250μl的乙醇（纯度100%）并吹吸完全混匀。不要离心。 将700μl的cRNA样品加入到一支放在2ml收集管内的RNeasy小柱中， 样品在4°C以1,3000 rpm离心

30秒。弃流穿液与收集管。

5. 将RNeasy柱转移至一新收集管并在其中加入500μl的RPE缓冲液(含乙醇)。样品在4°C以1,3000 rpm

离心30秒。弃流穿液，收集管则重复使用。

6. 将RNeasy柱中再加入300μl的RPE缓冲液(含乙醇)。样品在4°C以1,3000 rpm离心60秒。弃流穿液与

收集管。

7. 将RNeasy柱转移至一支新的1.5ml收集管中，直接在RNeasy滤膜上加入适量的无RNA酶的水（请参

考报告‖Labeling Efficiency-QC‖）以溶解纯化的cRNA样品。静置60秒，然后在4°C以1,3000 rpm离心30秒。

8. 将含有cRNA样品的流穿液放置在冰上，弃RNeasy柱。

9. T每样品取1.5μl用NanoDrop ND-1000测定产物量特异性活性(请参考报告‖Labeling Efficiency-QC‖)

?

?

?

? 在主菜单上选择MicroArray Measurement。进入Sample Type pull-down菜单选择DNA-50。 使用1.5μl 1x的标记液为仪器调零。 使用1.5μl纯化的标记的基因组DNA定量。测定A260nm(DNA)和A550nm(cyanine 3)吸光值。 标记的基因组DNA的特异性活性(pmol染料每μg基因组DNA)可用下式算得：

(Concentration of Cy3)

Specific Activity =——————————————— = pmol Cy3 per μg cRNA

(Concentration of cRNA) * 1000

Cy3的浓度

特异性活性 =—————————— = pmol Cy3 每 μg cRNA

cRNA的浓度*1000

* I如果量小于1.65 μg且特异性活性小于9.0 pmol Cy3每μg cRNA，则不要进行杂交。再次制备cRNA

51基因芯片_基因芯片

五、杂交

步骤: 1. 2. 3.

在10X封闭试剂冻干瓶中加入500 μl无核酸酶的水，振荡混匀。 水浴平衡在60°C

每个微阵列实验，将以下组分按下表所列顺序依次加入到1.5ml无核酸酶的微量离心管中。

cyanine 3-labeled, linearly amplified cRNA 10X Blocking Agent Nuclease-free water 25X Fragmentation Buffer 4. 5. 6. 7. 8. 9.

在振荡混合器上充分而轻柔的混匀。 在60°C孵育整30分钟以片段化RNA。

在芯片上加入55ul 2x GEx杂交缓冲液HI-RPM以终止片段化反应。

仔细吹吸以充分混合。注意不要造成气泡。勿在振荡混合器上混匀，在振荡混合器上混合会造成气泡。 室温下以13,000 rpm离心1分钟使样品从壁上和盖子上离下来并帮助减少气泡。 将样品置冰上并尽快上样至芯片。

Amount 1.65 11ul ×ul 2.2ul 10. 将干净的密封垫片基标记朝上与杂交室基座的矩形截面对准装入安捷伦SureHyb杂交室基座。确保密

封垫片基与杂交室基座完全平齐不翘起。

11. 慢慢将100μl杂交样品以一种“边拖边装”的方式分配到密封孔中

12. 将芯片慢慢放在SureHyb密封垫片基上，这样―Agilent‖标记条码朝下而数字条码朝上。确认这种三明

治排列。

13. 将SureHyb杂交室盖在三明治结构的片基上并滑动夹子将两片组装起来。 14. 将夹子手工紧固到杂交室上。

15. 垂直旋转装好的杂交室到密封圈上，估计气泡的活动性。

16. 将装好的片基杂交室放入杂交炉烤室内，设定至65°C，设定杂交旋转器至10 rpm。 17. 65°C杂交17小时。 六、芯片洗涤 操作步骤: 1. 2. 3. 4. 5. 6. 7. 8. 9.

预热足够体积的Gene Expression Wash Buffer 2至37°C。 把片基支架与搅拌子装在染色盘上。

将染色盘连同片基支架与搅拌子一起转移到磁力搅拌盘上。 用100%乙腈灌满染色盘。

T打开磁力搅拌盘并调节速度到4（中速）。 洗5分钟。

弃乙腈至合适的地方。 重复步骤2到步骤7。 在排气通风橱风干染色盘。

10. 用Milli-Q水清洗所有的盘，支架，和搅拌子。

11. 室温下用Gene Expression Wash Buffer 1完全灌满芯片染色盘#1

12. 将一片基支架放入芯片染色盘#2。放入一个搅拌子。室温下用足量Gene Expression Wash Buffer 1

灌满芯片染色盘#2以淹没支架，将该盘放在磁力搅拌盘上。

13. 将空盘#3放在搅拌盘上并放入一个磁力搅拌子。等到第一步洗涤开始后才加入预热的(37°C) 。 14. 从温育中取出杂交室并开始计时。记录杂交过程中是否产生了气泡以及是否所有的气泡均可自由转动。 15. 杂交室拆卸准备。

a) 将杂交室组件放在平面上，逆时针转动松掉拇指夹。 b) 滑动取下夹具并取下杂交室盖

c) 带手套从杂交室基座上捏住芯片末端取下芯片-密封垫三明治结构。尽快将其转移至芯片染色盘

#1并在此过程中保持芯片数字条码朝上。

d) 不松开手上的芯片，将芯片-密封垫三明治结构淹没到装有Gene Expression Wash Buffer 1的芯

片染色盘#1中。

16. 当三明治结构完全淹没到Gene Expression Wash Buffer 1后，仅从条码端撬开三明治结构。

a) 用镊子的一个钝头在片基之间滑动。 b) 轻轻将镊子向上或向下转动以分开片基。 c) 使密封垫滑落到染色盘的底。

d) 取下芯片并将其在室温下放入到装有Gene Expression Wash Buffer 1的芯片染色盘#2中的片

基支架上。

17. 当一组芯片全部放在芯片染色盘#2中的片基支架上后，使用设定4搅拌1分钟。

18. 在此洗涤步骤中，从37°C水浴中取出Gene Expression Wash Buffer 2并倒到芯片染色盘#3中。 19. 将片基支架转移至装有containing Gene Expression Wash Buffer 2的升温的芯片染色盘#3中。使用设

定4搅拌1分钟。

20. 慢慢取出芯片支架，芯片上的液滴尽量要少。取出芯片支架应在5-10秒钟。 21. 立即扫描芯片以防止在环境中氧化剂对信号强度的影响。 七、扫描 操作步骤： 1. 2. 3.

将芯片装在芯片托上。

将装好的芯片托放入到扫描仪传送带上。 确认单色扫描的扫描设定：

Scan region

Scan resolution (μm) 5μm scanning mode eXtended Dynamic range Dye channel Green PMT 4.

Parameters

Scan Area (61 x 21.6 mm)

5 Single Pass (selected) Green XDR Hi 100% XDR Lo 10%

点击扫描控制主窗体上的Scan Slot m-n，字母m表示第一个芯片所在的开始的槽，而字母n表示最后一个芯片所在的结束槽。

八、用Agilent Feature Extraction Software提取数据

操作步骤： 1. 2. 3. 4. 5. 6.

打开Agilent Feature Extraction (FE) 软件。 将待提取的图像文件(.tif)加入到FE Project。 设定FE Project的属性。 检查提取设定参数。

选择File > Save As 和浏览以保存FE Project(.fep)至所需位置。 选择Project > Start Extracting并导出数据到文本。

实验流程：

第三节 实验结果

1.总RNA质控结果 提取的总RNA经琼脂糖凝胶电泳分析，可以观察到18S、28S

两条条带，有较好的完整性 (图1)；RNA样本经紫外分光光度计测定OD260/OD280值均在1.9-2.1之间 (表1)

表1 RNA定量和定性分析

Sample ID

1 2 3 4 5 6

OD260/280 Ratio 1.96 2.01 1.99 2.00 1.98 2.01

OD260/230 Ratio 2.40 2.42 2.42 2.41 2.39 2.32

Conc. (ng/μl) 550.80 999.21 700.44 703.95 601.95 751.78

Volume (μl)

10 10 10 10 10 10

Quantity (ng) 5508.00 9992.10 7004.40 7039.50 6019.50 7517.80

QC result Pass or Fail

pass pass pass pass pass pass

*OD.A260/A280比值（1.8-2.1）应该接近2.0保证RNA的纯度.OD.A260/A230比值

大于1.8

Lane 1: Total RNA of sample 1 Lane 2: Total RNA of sample 2

图1 总RNA电泳分析

2. 差异表达基因筛选 基于基因芯片的原理，我们得到的数据是每个基因对应探针的荧光强度，为了消除生物学系统误差，经过修正，我们得到最终的Normalized值(表2)，然后在Excel中取两组数据的比值Fold-change(图2)，即normalized intensity of treat/normalized intensity of control，即F/N。根据数据的信息量，人为设定筛选标准：Fold-change>2或<0.5认为2倍以上变化的基因即为差异表达基因；Fold-change>5或<0.2认为5倍以上变化的基因为差异表达基因；Fold-change>10或<0.1认为10倍以上变化的基因为差异表达基因。此外，还可以做对数处理后比较|2-Fold change|>1，|5-fold change|>1，|10-fold change|>1，界定为倍数差异。2-Fold change=log2（normalized intensity of treat/normalized intensity of control），5-Fold change=log5（normalized intensity of treat/normalized intensity of control），10-Fold change=lg（normalized intensity of treat/normalized intensity of control）。

表2 正常肺组织和肺炎肺组织差异表达基因部分数据 Group-Normalized Intensity

[N](normalized)

708.228703 0.521212.646933 878.467253 524.253497 1784.979700

[F](normalized)

298.436450 576.614820 381.553203 168.587817 367.486113

Gene Name

PER2 BAHD1 KIAA1026 C11orf21 FZD4

7857.677800 918.384633 448.655283 678.663100 5593.953233 1179.106667 1781.639533 755.539400 544.475217 1002.827517 1611.357933 1086.207667 3606.139200 830.409683 877.365277 693.287933 3488.770900 332.552350 2304.095867 960.115020 866.059433

2993.335933 188.512253 207.171807 282.442587 228.511870 247.403737 565.994190 222.343023 221.416447 270.869663 792.378363 340.754660 518.236183 307.914230 222.736603 324.037160 1569.500533 131.972063 780.259453 437.600890 284.219620

HEG1 SEMA6A KIAA1450 PLCE1 AGER AKNA ATF3 KIAA2002 N/A OTUD1 RXRB GIT1 ALPL CUGBP1 LST1 LST1 LILRB1 LILRB1 CFLAR LILRB3 TNFRSF10C

图2 F/N比值

本文以2-5倍变化的基因，介绍如何做heat map。选中F/N这列数据，“开始”-“排序和筛选”-“筛选”-“数字筛选”-“介于”，即筛选出满足条件的基因

51基因芯片_基因芯片

图3

筛选

图4 数字筛选

3.做heat map图 由于[N](normalized)，[F](normalized)数值较大，因此所有的数据均除以1000后，在以2为底，取对数。选中数据，“开始”-“条件格式”-色阶-“其他规则”-按图6设置规则-“确定”，得到图7，下一步去掉里面的数据选中数据，鼠标右键选择“设置单元格格式”—“数字”—“自定义”—半角状态的分号“;”（图8），即得到所谓的热图

图5 打开色阶设置

图

6

设置色阶规则

图7 图8 图9 热图

第四节 结果分析

为了解参与肺炎发病过程的基因变化，我们应用现阶段较为成熟的基因芯片技术来分析基因的表达水平。我们发现肺炎标本相对正常肺组织上调2-5倍的基因有1231个，下调2-5倍的基因有1685个(表3). 上调或下调的基因使用Go分析，做功能分类，再结合上调或下调的基因水平，最终选择可能的关键靶基因，进而往下研究该基因的功能以及调控机制等。

本实验成功地将生物芯片技术和生物信息学完美的结合，为处理芯片结果提供了方法。

表3 肺炎下调和上调2-5倍的基因表达荧光强度值

F vs N_down

[N](normalized) 708.228703 1212.646933 878.467253 524.253497 1784.979700 7857.677800

[F](normalized) 298.436450 576.614820 381.553203 168.587817 367.486113 2993.335933

Gene Name PER2 BAHD1 KIAA1026 C11orf21 FZD4 HEG1

[N](normalized) 167.130373 251.518353 293.551423 1541.180033 3280.387433 204.278343

F vs N_up

[F](normalized) 449.269827 692.628853 709.893110 4579.679133 7173.687800 491.264177

Gene Name CLCN3 CGI-09 AHCTF1 CCT6A C18orf10 ACP6

448.655283 678.663100 1179.106667 1781.639533 755.539400 544.475217 1002.827517 1611.357933 1086.207667 830.409683 877.365277 693.287933 3488.770900 332.552350 2304.095867 960.115020 866.059433 2490.080667 500.586767 4088.582500 329.301087 2258.420200 548.122307 1057.918020 267.296497 506.113610 999.275300 883.595480 1108.917057 401.629337 1200.684300 764.171513 17389.423333 2999.430433 481.336060 1441.446500 1316.641200 892.125307 1454.336733 748.477090 7565.505667 490.339827

207.171807 282.442587 247.403737 565.994190 222.343023 221.416447 270.869663 792.378363 340.754660 307.914230 222.736603 324.037160 1569.500533 131.972063 780.259453 437.600890 284.219620 946.676317 207.351660 1686.117067 149.382741 477.672377 224.276710 247.968450 131.652313 204.700435 219.049610 396.933330 266.070873 179.680350 554.959853 347.972753 5331.480233 1475.052933 234.678397 548.639500 551.989533 347.808580 723.158203 251.486640 1740.381900 202.811937

KIAA1450 PLCE1 AKNA ATF3 KIAA2002 N/A OTUD1 RXRB GIT1 CUGBP1 LST1 LST1 LILRB1 LILRB1 CFLAR LILRB3 TNFRSF10C CCRL2 PPT2 DLC1 NCR3 LDB2 CROP NDRG2 ACSL6 N/A EDNRB ZNF589 N/A SLC8A1 WDR23 ZBTB20 CAV1 OGFR ERBB2 184.574647 RGL1 PARD3 TXNDC6 SLCO2B1 N/A SLC17A5

680.290587 1612.569733 MGAT5B 710.094577 1957.932100 TAF2 148.930183 367.811710 CASK 286.756860 651.940423 GMDS 168.762913 416.377990 CSNK1G3 641.010833 1868.711667 SEL1L 232.095320 601.694743 LYPLA1 1207.086167 2423.328800 FLNC 433.770100 1834.349200 N/A 475.683410 1222.616467 GPR89A 460.361423 930.370733 UCHL5 416.960733 1936.623967 GLS 587.135010 1325.902700 C14orf100 714.617630 1464.055467 PSENEN 2365.571500 4835.081500 MRPS21 231.369550 724.393467 ANGPT2 1123.836000 2550.662200 CHPT1 154.577077 742.623543 TMPRSS4 889.893683 1824.437533 CEECAM1 505.503767 1268.328767 C1orf121 404.140097 1058.850133 MRPL39 1857.106833 3962.156333 C6orf149 2107.248433 8547.151133 SEMA4B 197.431277 489.768120 CLSPN 559.750663 1677.731500 VEGF 1460.700567 6532.096000 RAB34 282.127833 734.366600 RP11-34P13.4 149.670760 567.392540 LARP4 482.731087 1123.932800 OSBPL3 283.378333 1066.142000 HYAL1 220.722057 669.133453 HYAL1 148.770170 430.697027 GRIK5 256.958697 731.381393 N/A 404.227067 1411.529667 N/A 487.332560 1424.910033

HSP90AA1 438.641670

C1orf27

157.290150 446.645533 ROD1 235.646540 774.333610 KLHL5 344.991247 1240.201967 BXDC2 961.246790 2059.885167 KIRREL 542.136517 1368.248133 CTNNAL1 249.014113

585.799697

EDEM3

1894.097167 399.024780 1809.399467 1398.686267 2679.396267 1081.612767 1148.058700 807.357517 1455.661433 614.526767 1133.369233 1019.509647 397.600810 1242.761783 1117.346133 316.938227 1184.711633 832.441833 1513.069533 341.749757 1272.617100 2878.239567 1731.462867 1170.143167 1835.592767 3881.103767 568.637367 786.243930 530.372510 3151.160000 615.149990 1033.933363 2375.591633 992.435967 745.270263 433.706903 1193.802000 828.705333 887.017300 400.653240 6717.046400

593.348490 162.592447 809.302827 574.981823 1096.470620 390.808193 448.735100 347.548847 560.712447 299.761857 285.301383 257.570450 163.332513 555.613107 533.472563 138.559210 546.035483 377.918303 573.658933 161.435013 570.512860 719.858167 727.603580 541.457657 785.631333 1787.622333 189.478550 184.373380 232.558608 1466.541467 199.895609 364.291557 827.811867 412.386957 251.854469 199.172833 579.809507 327.941520 402.307643 170.837063 3231.655533

N/A HES6 NALP2 RABGAP1L LILRB1 PEO1 NOTCH2 DUSP2 BBC3 GTPBP3 CD300LF CD300LF MAZ HOP TAC4 BRSK2 BCL2L13 FBXL10 SVEP1 SLC35A1 N/A LIMS2 BEXL1 N/A C8orf60 NOC2L C14orf159 MOV10 FYCO1 FLJ11292 CNNM2 273.430543 SELENBP1 CRYL1 FGFR1 ANKRD15 PQLC1 FLJ23569 TMEM25 CRISPLD2 TMBIM1

258.865460 700.820947 162.728302 415.993737 1508.193700 3164.896467 419.042193 931.797153 215.331380 511.032273 471.578457 1086.842780 216.965937 674.790450 693.161653 2631.310967 200.754548 794.300983 339.660870 815.434437 364.029763 1056.838267 201.923773 434.310577 512.140147 1340.845100 379.323293 821.375567 808.282100 2325.867100 624.611247 2195.370933 349.707667 1089.212000 128.123210 477.258010 279.467537 682.893167 199.916827 542.448860 282.570290 665.173900 1162.519357 4142.522033 842.114700 1859.837200 510.628210 1212.780433 1434.661467 3776.161967 185.113727 655.828347 680.684517 1669.229367 134.876417 368.964797 133.205200 287.640500 1509.351033 3604.043200 738.280217

2699.590733

563.972677

N/A

279.524863 855.724413 823.425867 1685.160533 179.375690 739.096940 224.352560 486.473177 209.926800 452.623803 303.940800 1518.456933 317.137780 1140.024333 177.315233 357.031950 1317.080400

2896.517933

MYEF2 NAG6 CDH24 MAK10 TFB2M C20orf119 OACT2 FADS2 NLN N/A TTC9B KIAA1641 TMEM79 N/A EFCAB4A BACE2 IGSF8 SACS ADM2 N/A KIAA1924 PROM2 LTB4DH FLJ36031 ZBTB38 FLJ37464 ZNF185 SGPP2 ZNF93 FLJ22184 EFCAB4A

GPR39 LYPD1 EGLN3 ATP5J2 N/A SH2D5 N/A N/A RUSC1

1118.211233 768.295783 2263.574033 933.114733 462.025107 631.062687 2336.996733 2238.233000 513.890713 5207.276233 1279.396300 938.283427 451.716010 553.098203 780.863483 1192.311033 1219.129833 2252.237867 7538.124667 395.692100 467.739780 397.712443 851.879767 1216.193417 366.747207 5006.989033 603.130277 4809.116500 567.941020 6259.108833 363.049923 958.269317 548.968370 1859.825333 455.105057 1086.130433 2026.922333 15936.199667 2285.153533

523.144230 191.315957 1041.520220 320.979900 185.674203 238.536333 897.288000 870.412057 249.177000 1329.939800 332.845157 408.270630 213.500057 227.060537 156.886498 409.148823 508.263203 991.081200 2842.369433 182.231917 172.847460 190.904490 248.202277 586.708023 153.565387 2200.665733 246.435670 1941.070667 221.090073 2778.477633 174.585193 198.659973 137.379081 595.095250 211.575613 410.651307 635.980967 7954.060500 852.891233

PLXNA2 BTK RNF166 CD37 BCORL1 TINAGL1 GPR177 APOB48R C2orf18 ELN AMICA1 N/A PLCD1 OSCAR C14orf159 CCDC78 CD300LF OLFML3 GSN LOC283849 FUCA1 DOCK8 VIM LOC440104 FRMD3 N/A LHX2 C1orf38 N/A C2 DAB2IP FLJ20701 RASGRP4 LOC401622 LOC201164 GCAT N/A N/A N/A

173.192667 1598.925767 182.929103 194.919860 224.240003 213.195673 321.436363 173.398740 3318.052067 366.765053 696.424947 727.324617 222.623887 854.130773 409.100770 550.270683 299.233630 335.985137 730.495733 1368.810033 1219.749720 1495.675533 329.316550 7115.864333 993.692137 225.272220 574.362487 336.829023 367.751160 157.753640 241.233763 405.435750 217.796710 306.598970 918.684983 1406.715800 120.922663 290.801367 374.516170

601.725933 3333.964733 427.938087 557.423513 613.161110 723.900520 925.548440 398.806537 7086.525600 925.817780 2156.478300 1472.232767 466.835270 1942.691233 859.882233 1116.013533 601.851933 693.274600 1769.880833 2999.803800 2457.207767 3213.372300 1130.436800 15779.772000 2288.614367 483.084350 1230.612067 746.370617 1319.110967 385.101150 592.309533 1509.019217 717.214197 1300.137800 1894.800900 2815.459667 458.814433 702.885233 1006.287517

N/A DNM1 N/A GRAMD1B N/A N/A N/A ANKRD50 AEBP1 DNAJA5 N/A CAPN5 C1QTNF6 TPM1 TRIO N/A ATP9A GPR89A GPR89A CDH24 N/A MICAL-L2 STRBP CAMTA2 DDX27 CKAP2 DNAJC10 GLI4 NDUFV3 EVC2 GLYCTK RAI14 ARHGAP19 RAI14 PSMD6 TARSL1 RBM24 U2AF1L4 NR1I3

51基因芯片_基因芯片

853.559930 958.429217 1620.118000 1051.237750 586.463910 1092.088067 1757.719800 1085.535767 458.393140 555.850807 705.129730 495.675660 1219.230610 1588.962433 1081.876800 511.627460 514.589133 1213.863300 1157.962483 879.355967 1205.048767 995.035653 1155.998153 780.138267 384.033310 1083.837920 1799.714667 306.858780 511.072613 1127.133233 2575.001967 726.429500 1737.530833 3674.146533 772.070163 1945.857567 320.866500 510.572267 1283.771833 568.822007 490.964060 338.032913 881.125443

337.572123 239.401687 615.916820 487.314657 265.648360 484.093503 788.419217 450.865580 187.154810 220.857987 253.704963 216.457130 330.968033 780.653430 275.813793 248.921407 256.021207 396.045960 536.217550 306.759330 417.753660 464.710183 422.547320 260.647313 173.882280 506.981067 642.753733 149.677100 239.048643 495.616600 980.820067 321.691073 543.113077 1550.543267 210.412428 434.414830 126.598547 144.582950 479.317357 219.738603 188.852105 139.304473 319.050037

NDRG4 N/A FLJ42200 N/A N/A IL16 TNKS1BP1 N/A N/A N/A DENND3 N/A ADCY4 N/A NDRG4 N/A WHDC1 N/A N/A MDGA1 SLC30A3 SLC30A3 N/A PDE4B N/A N/A MACF1 N/A KIAA2002 NSUN4 N/A NEIL1 TESSP5 CIRBP N/A CYBRD1 ZSCAN2 C20orf151 N/A N/A N/A N/A N/A

1139.147967 1537.646400 797.352853 344.572637 1693.610200 587.738557 734.338690 5650.908767 1725.543233 282.055263 249.164047 905.801713 291.531070 965.105833 522.082927 226.472977 3067.420667 254.347080 604.944963 1751.592567 1479.254267 907.166810 344.563790 761.495240 951.011133 555.730133 457.082530 1002.413753 413.395650 3855.504033 563.984353 307.600947 757.982967 191.714223 300.485800 1629.196800 184.674313 420.611783 2495.219467 539.217907 120.294427 1692.908467 407.432143

4798.507100 5790.705867 3163.607233 951.837613 3510.150233 1309.740600 2070.930600 13930.550333 4557.951000 676.560367 645.229250 2096.290533 644.287337 2176.065967 1415.556820 837.975917 11423.140000 743.441907 1225.173767 3801.462567 4763.649433 1966.569933 796.884437 1827.892733 3533.887767 1237.099200 1046.964133 2151.642167 1136.691687 8821.420667 1573.239033 808.715843 2020.221733 480.118097 756.833700 4496.553333 400.168660 1004.199333 5136.922367 1382.549600 265.527853 5397.697867 914.190540

CDA CEACAM1 IGFBP3 P15RS EIF2S2 CINP SRP54 TIMP1 EIF2S2 YEATS4 CA12 ALG8 DDX18 C14orf11 IFRD1 ACTL6A UPP1 ATP6V1D NCBP1 C14orf166 SNX6 HIG2 SMAD1 AURKA CBR1 CENPA EIF3S1 S100A2 SAR1B PSMA6 STX1A GATA3 USP14 ZNF24 ADK PITX1 FST CHEK1 JTB C9orf64 PRO1853 CEACAM6 GMNN

881.188610 409.061380 793.449407 549.649103 835.222337 4675.616633 1040.782733 1355.297567 342.888563 1416.266700 2195.011567 1475.485767 1035.823243 873.514980 2331.928533 2742.861267 2401.875000 509.037420 1517.748900 3874.626967 1602.692833 8217.288100 1347.721967 828.051333 635.605403 518.028310 457.158977 1165.707400 376.365377 833.364990 1177.080200 1670.367800 1146.756000 5254.482067 1322.959800 2100.895167 437.542053 548.303250 679.954783 6724.409333 1563.783400 583.201913

257.617610 176.962127 240.868660 230.569407 407.686860 1427.229100 406.888863 614.297750 151.822642 669.107260 1078.077900 503.956087 294.885010 428.688337 759.444610 1332.656800 972.573717 233.508613 408.516760 976.493887 674.903857 3610.249833 425.562117 282.514527 167.611437 214.205113 223.076257 507.050537 146.259683 255.124840 528.829043 428.620567 450.172527 1352.119867 448.578917 527.894533 212.422503 160.475450 339.088590 2155.923633 584.737290 191.675173

LOC15076

3 PXK AEBP2 SERPINH1 ERBB2 LCP1 CD83 CKMT1A CYYR1 CYFIP2 JUND DNAJC11 359.869033 L3MBTL2 ELMO1 MOSC2 PPM1M NDEL1 SYNPO2 N/A N/A N/A ATPAF2 ESPN ERG GZMH ZSCAN2 IL16 N/A NPNT SIGIRR N/A SIDT2 MGC45438 PLXNA2 RAB11FIP1 IZUMO1 PDE4A MLLT10 SERPING1 CCL4L2 CCL4L2

144.947730 543.701253 1024.628720 2686.097733 1026.561913 2080.847567 3239.157733

7073.990767 361.021280 1171.169783 149.224228 436.188150 294.373140 679.700100 390.950727 1112.373500 234.463237 512.044297 153.440697 387.900373 1038.067333 3357.643100 2565.915233 5430.404867

844.615467

SAA1

300.273503 788.489103 776.756920 2288.539133 641.692033 1531.998233 1567.495100 3620.342367 1506.096867 3360.792400 1331.267833 4186.240000 1378.122167 4270.855933 121.495313 443.578073 524.163630 1337.793733 724.030233 2362.544433 583.198180 1539.037233 1539.633433 4315.559700 265.955240 597.641280 518.184387 1279.169900 406.303833 888.763880 4350.475167 11324.677233 496.665647 1355.822567 999.631257 2219.246267 2089.028367 8233.909000 608.477923 1571.792567 455.278763

1079.093683 408.347670

886.536620

1313.265600 2628.318467 115.765400 307.145203 456.024807 1407.000053 1267.007133 2769.568733 2134.003900

4529.488667 2848.092333 5881.835900 366.333120

820.137167

CCNH TXNL2 PAIP1 CYCS GMNN HYAL3 SIKE OVOL2 C15orf41 C1orf170 MGAT2 CCT5

UBE2V2 TPM1 BCL2L13 EIF5 EIF2S2 SPHK1 C1orf43 CAP2 CHI3L1 PTPN11 FADS2 PITX1 SMAD5 KIAA0523 PGM2 TIMM17B LYPLA1 SUB1 TARS SLC38A1 GALNT14 LOC404266

PWP1 CSN3 CHRNB1 CFL2 PRKCDBP FANCG CA12

1745.671100 1319.378267 19217.845333 755.261013 444.630367 1122.475020 1801.074433 5784.022733 8717.927900 4563.502433 24208.064667 5707.308567 1162.166817 514.457460 1206.251800 1579.637833 7136.967600 687.756033 1971.731733 697.055220 876.208950 4179.346367 1987.889400 1486.603733 607.945823 2355.727133 1503.676100 1916.086600 6662.677567 5647.489167 1266.058867 1114.443133 1225.236033 2986.481267 7459.151467 1371.871300 10442.438333 3097.384833 1583.639633 707.949077 608.006333 1010.705100

453.432590 422.749560 7699.190000 292.670897 212.629550 341.639570 806.374633 2645.981933 4092.399267 1344.020933 9192.540000 2775.162767 521.687887 213.590433 387.340467 723.029887 2392.972833 195.027603 924.685300 242.787547 222.175763 1632.165600 686.835567 465.240447 291.553163 712.129233 627.435750 934.658043 3017.631867 1510.855367 629.618663 423.677553 606.479593 1410.464333 2173.171133 325.537200 3581.859800 922.246410 442.424700 192.290313 280.371307 410.425527

CCL4L2 CCL4L2 HLA-DPB1 ATP13A2 GSTM3 FAM53C GGA2 EWSR1 ITM2B CD52 S100A4 CIRBP N/A BAP1 NCF2 WDR8 CTSS TMOD1 DUSP4 MED18 KLK10 DHRS3 DAB2 MUTYH FTO ARRB1 ATPAF2 SKIP DHCR24 FOS DOK3 C16orf30 NFYC DUSP6 MGP FPR1 DCN SCD RUNX1T1 PTN VAMP3 APEG1

161.367210 168.216833 794.450833 169.005477 209.295430 281.259133 1101.581543 140.412102 742.412417 409.512503 146.966805 167.612023 820.913367 204.891000 679.327200 241.561957 1990.998133 656.278390 1408.604400 1028.161067 221.565280 934.078687 551.666037 179.526797 547.420493 1052.641720 807.456920 1916.597433 678.156767 261.466413 416.998053 513.177890 1602.731883 691.839133 7965.964800 1996.588733 2661.413600 448.747627 154.917417 179.007997 2015.667533 1212.323433

646.139367 375.278600 1640.905733 405.518973 480.020723 578.708063 2569.512533 308.221120 2090.780733 1103.810627 324.028890 435.214457 1725.709400 503.746620 1394.925867 1171.048233 9002.867667 1371.468267 2821.024267 2359.729800 485.804633 1966.187067 1530.433600 474.315873 1253.894543 2885.905667 1719.274600 4862.240233 1760.507833 591.030670 1221.064857 1313.501400 3715.148500 1417.932533 18772.231667 7452.559400 6825.911800 1019.147857 373.617590 415.470597 7768.197667 2442.833733

STEAP1 FGFR1OP CYP1B1 SLC7A11 DNAJB5 SDCCAG10 GMPS GUF1 CLDN1 C21orf6 DAPP1 ERO1L PMAIP1 PREPL CALU KRAS PLAU MGC21675 METAP2 C7orf24 SLC25A36 ZC3H11A CHMP4C MYL6B BACE2 CEACAM1 ACTR2 SLC25A37 ZBED1 AASDHPPT HDGFRP3 RAB3IP HOXB9 EPS8L1 ACTN1 LTF LTF USP25 TIA1 AASDHPPT RAB34 LOC133619

1482.938267 4856.585300 1150.319967 384.976270 711.733450 1804.221567 517.605323 124.261785 SAPS2 FABP3 HVCN1 523.006593 578.812080 330.807423 1066.176433 1719.799200 733.135310 682.072353 SSR3 LYPD1 HIST1H2AC MB MGC16186 162.081630

1010.315833 1709.244900 4616.072333 27994.475000 6674.328133 1611.361667 2270.593433 4812.438367 3261.081433 734.619267 23078.561333 682.561710 2213.939300 670.033323 563.073750 4440.802833 929.359023 27113.212333 26113.931667 33701.981667 418.425537 954.278633 1520.833267 514.852600 7282.778133 740.617783 1169.355423 1703.922333 15687.173667 2957.844067 13137.354000 3531.105633 957.014920 309.566837 9967.582333

466.498097 697.935733 1644.062633 13568.619333 2831.335567 780.302467 603.939733 2318.615300 1402.820233 338.238403 10633.712667 178.801123 792.586100 329.711063 248.101237 1302.785967 241.790740 8886.318333 12961.864333 16715.767000 192.106170 344.804433 568.960133 235.118363 2513.467467 324.814487 489.473307 653.910023 6105.685533 942.293143 6359.193100 814.688233 431.446950 137.943003 3283.788233

APOD PLCG2 LCP1 HLA-DRB1 FSCN1 STK11 MAOA LITAF FBXO7 ISOC1 HLA-DRB1 NDN ITGAL ANXA8 ZNF486 GDF15 C5AR1 C1QB HLA-DRB3 C1QC MCC ZNF331 PPM1M HDAC11 EMP2 GNG11 HVCN1 SF3B3 HLA-DPA1 CENTD3 GLUL FLI1 CSTA FCHSD2 CLU

215.432627 193.797133 297.986333 495.986060 285.778557 1098.074110 540.359110 256.701700 620.749547 340.004393 838.896457 2227.757833 194.390040 123.728227 1592.744567 423.502920 614.341247 219.457303 641.806003 139.595342 261.518233 337.308593 2624.248267 300.522393 251.407710 301.581510 322.602900 168.516047 417.178620 442.111480 391.344863 350.999193 3332.266767 1585.466967 9517.142000

576.887157 524.901943 997.902500 999.064133 748.094577 3525.074867 1465.126067 719.653570 1485.884713 884.699167 2573.699667 5072.280033 616.024880 284.800147 3390.805867 979.659537 1628.104200 448.080073 2409.298067 417.214347 525.951830 990.505667 5618.375467 655.350707 596.549967 892.325363 771.538247 372.149637 1158.414123 1134.993533 876.172477 1004.124467 7676.270233 3512.797933 32126.745333

RND2 SNX7 SUB1 TMTC4 FBXO5 FBLIM1 SRPX2 SAA2 FKBP3 SULF2 BNIP3 C18orf10 POPDC3 GDF1 SNRPA1 YME1L1 STK17A AGPAT5 CHRNB1 ANKRD50 5-Sep ATP6V1D IGHG4 FLJ13195 HRB2 SCCPDH AP3M1 C15orf29 BZW1 ADORA1 MITF PRPF40A TJP2 MTPN COL3A1

5651.440600 16571.915333 282.302403 799.953340 2034.965933 1372.752300 2383.430033 1856.253533 412.728580 11710.731667 699.244983 4714.841300 293.505010 477.740407 565.873043 399.332373 1417.769433 17935.043333 1657.512767 589.922783 1153.791013 305.263873 1119.853500 3974.069000 1164.881400 1490.218600 6199.040800 5484.517667 894.153573 379.541977 2357.889300 1854.472300 3985.967167 1049.015353 2342.781633 297.019563 1256.287533 2028.038200 1264.094467 1465.850533 2394.383067

1803.328200 6795.985767 131.537923 367.070683 408.129490 534.728607 1126.753733 835.576900 168.361013 5399.764767 278.396017 1226.163967 136.409097 227.175060 193.782237 129.367987 676.696070 8663.601333 723.993077 162.226722 575.304913 138.823497 461.117973 1020.104590 458.805440 683.617420 2945.251033 2339.086867 288.469430 184.093950 1004.537430 866.342900 1583.522267 514.182193 913.595713 132.130977 315.271507 412.158210 568.112017 447.089517 1008.997877

STOM GSTP1 FXC1 NDRG2 LPL FAM105A GPNMB TCOF1 SORBS2 CD9 MRPL44 HLA-DQB1 ZNF673 HSD11B1 CFH SMPD3 MGC7036 HLA-DPB1 RECQL4 KCNJ15 TTN CLDND1 NEIL2 SNCG DNAJC11 N/A TIMP3 CYB5B ABHD10 FBXO8 TPP1 INPP1 PRG1 RENBP SERPINA1 495.393787 NKG7 SDPR ASAH1 CCDC69 ASAH1

483.037017 1036.566270 MUC20 549.294923 1777.858700 C14orf24 289.941003 1161.433390 DCBLD2 1133.923313 2833.690167 FBXO27 670.354037 2179.828000 SLC22A18AS 875.076213 2052.610000 TYSND1 425.392903 1114.941167 ATP6V1D 549.739257 1508.772667 MGC24665 3029.329033 6141.369100 MGC10433 232.457167 638.883923 MRPL50 2417.520200 5788.027333 AXL 320.543863 793.737333 BATF 283.393253 633.703693 LMAN1 989.469690 3883.709900 AQP5 625.660733 2310.317567 PPP2R2C 220.607477 445.777780 SLC12A2 549.750253 1384.847267 TCF19 691.493300 2026.283333 FER1L3 606.251423 1794.113567 N/A 281.621100 675.240490 ATP11B 1185.903867 3114.522233 GPR137 216.186470 514.380197 LPGAT1 312.453320 849.030353 PPP2R5E 429.258117 924.713390 P4HA1 866.667220 1951.832200 GPR137 835.615813 1950.277933 MX2 136.511305 278.310120 NPTX2 184.170497 496.555040 MAPK6 260.462067 872.400153 FAM72A 207.801300 479.163693 RPS6KB1 636.113403 1638.805800 ACTL6A 251.671440 630.485333 FGFR1OP 257.824803 702.736267 C9orf100 646.625603

1363.570300 N/A 548.171217

1112.378500 SART2 1388.839833 MGC24665

465.156107 2032.643633 SLC39A6 225.697767 547.245553 C9orf40 227.340217 707.181920 DDX6 294.686930 668.527750 NRIP1 312.437983

1287.357000

KIAA0802

51基因芯片_基因芯片

1488.339533 634.770233 392.888153 6911.733067 4740.531567 7952.073233 506.146757 3453.795733 680.497667 956.332967 378.348807 770.616667 296.370820 1206.490533 2529.139300 325.417410 2456.965733 1313.352233 1380.405733 593.404267 553.138557 1487.677533 810.512203 740.341613 1262.401933 2051.161467 1078.533933 687.459867 959.257937 2216.724633 1349.373700 879.755420 721.920307 3439.839833 1836.551167 4084.593300 708.747913 4494.500733 715.370733 1709.409233

318.935237 206.285720 170.749333 2250.067800 1864.570167 2409.426000 204.480383 984.432380 285.768507 423.207757 178.402693 253.647167 132.207738 580.445223 1167.596750 154.493107 994.413950 390.043867 521.707233 205.570073 237.240687 513.256573 210.811100 342.175410 355.583103 677.845080 264.233650 203.279693 287.076773 934.130467 546.553183 300.396300 360.756110 1455.500700 823.886303 1205.762383 351.252700 1299.610533 344.192050 467.759437

LOC35290

9 CYP4F11 ETS1 CXCL16 TREM1 NAPSA PTPRC FCGR3A ABHD2 C9orf19 C1orf102 C14orf58 ZNF169 ABHD2 FCGR2A IRF2BP2 C8orf4 FCN1 GIMAP4 NKTR SLC16A4 C9orf61 VWF GBP2 CPM AQP4 389.366210 SPOCK2 MTSS1 TRAF7 KRT5 AOAH ARHGAP30 CD4 NPLOC4 HLA-DMA ARHGEF10 HLA-DMB CNOT6

TMEM16A 434.669327 1751.605867 OTOA 403.907340 947.924377 HBS1L 214.587740 459.926353 SCAND1 720.193877 2430.003400 C11orf43 232.215257 644.681677 KIF3C 6513.470333 16609.929000 COL1A2 1001.465467 2308.029300 C21orf45 190.832323 626.519590 PPP1R3B 321.538693 741.628590 MAN2A1 1958.135133 7337.137033 COL5A2 512.376407 1246.081113 KIAA0391 282.515010 766.141753 ROD1 1139.753583 3409.524200 TAF15 2259.331167 9947.647000 UPP1 2649.749467 5436.887967 ZDHHC12 387.050667 1234.850387 bA16L21.2.1 290.460977 622.100467 LRPPRC GALNAC4S-6275.562337 767.916803 ST

220.322563 454.221527 HIST1H2AC 239.787410 659.893393 GABPB2 293.945180 886.298217 ATP10A 461.655823 1152.960357 RANBP9 594.110963 1593.676433 IGF2 293.982283 635.721057 RAGE 7091.440433 31280.972333 H19 141.856540 705.846590

EDIL3

1053.372613

HOXC9

717.599020 2960.236367 CDA 166.641557 362.484847 FLJ22624 630.013603 1828.971833 OTUD4 213.091020 655.842133 FLJ14803 579.710623

1245.953033

DERL3

413.746033 892.440270 WDR62 2447.673867 7964.945000 C19orf33 162.623830

367.637877 SCOC 353.462993

809.983800

LARP4

344.840127 1074.539533 PPIL5 653.495383 1457.178467 DTX2 424.299563

1368.593600 MMP14 615.094347

2796.957533

CDKN3

628.135190 1198.537933 1110.794633 6554.471433 1453.399533 1297.965700 2475.412833 1517.289967 2048.527867 1499.699767 538.588423 578.222467 810.486513 16252.019667 1210.631667 20402.872333 2011.813567 1000.635167 1796.873033 340.956953 956.884900 6416.459433 5313.487633 974.227633 1180.337767 1530.237933 8491.920800 928.885333 1517.672733 1634.162900 5571.037800 502.119720 4478.088600 2287.297867 718.848587 739.353187 1027.747433 755.523453 1834.278867 665.144007 1091.142620 733.855117 933.066833

217.325457 486.814343 513.759173 2347.560667 343.142397 644.511543 631.161743 403.892220 984.640227 616.891593 260.091257 227.382165 299.507143 7340.751000 277.500133 5462.683467 508.467710 274.217113 825.228733 167.322017 412.779340 1998.629667 2405.512667 422.320853 480.839507 481.683240 3124.999633 426.013183 408.192027 654.498297 1338.557667 152.922030 1970.832500 1063.778167 297.622233 290.304107 507.222740 258.816163 749.486473 174.440067 337.171733 355.437553 404.704900

MYO15B LAIR1 CCL21 TPSAB1 CD93 IL10RA PCDH17 CD300LF PIK3R5 RFX2 CBLC MGC45491 C2 SELPLG SNX25 C1QA N/A PIK3R1 CD83 PIWIL4 PHF17 PRELP LTA4H SAPS2 HVCN1 CYBB GPNMB

EIF4ENIF1 TMEM16A SIRPA MGC45438 FGL2 FCER1G RHOBTB2 ENPP2 OSCAR THRA CRY2 CIDEB SCNN1B MEIS1 RPL3 TEX2

1100.222023 686.520800 584.855850 413.953927 1107.957767 241.897023 344.591780 259.433600 684.092100 817.148607 141.756430 363.992570

688.707800 591.670443 420.213687 468.758033 988.899887 220.598338 183.186960 476.451907 226.263850 127.016243 271.262587 151.290793 239.024993 1803.370167 880.067667

120.035600 249.450630 214.470967 1169.031300

1267.837267 596.281640 2084.922067 261.917280 232.059797 1298.405733 192.468370 907.085743 208.267467 218.268743 336.728450 335.037907

2289.470167 1803.926033 2364.911333 861.837383 4651.737033 755.331763 1546.472300 879.012120 1840.734833 1754.745900 427.401773 1142.467817 1450.895600 1863.812467 1692.692033 1268.545267 2591.411200 531.494377 501.462380 1308.664367 503.464867 452.941407 591.315533 418.258767 526.621213 3804.344100 2614.622033 493.675193 616.929843 437.614500 3674.404867 4311.139267 2480.783000 4877.012267 611.076377 629.222157 3063.894000 517.880337 3690.886767 518.338827 498.208260 735.346413 1517.759000

LOC144501 GTF2H3 THY1 CLCF1 RAB34 N/A SULF1 CD2AP KRTHB3 CARD9 MGC4677 C7orf13 DHFR HMGB3 PRDM8 FJX1 C14orf169 CHORDC1 C17orf42 N/A HOXB2 LOX PRRX1 PSD3 RAB11FIP2 TBC1D3 TMEPAI LOC645009 APBA2 LOC554249 C14orf78 AMY1A CIP29 DTX2 WHDC1L1 SLC6A14 HIG2 PPP2R5E C18orf23 KCNK15 RND2 IDE NETO2

575.208477 1134.990817 679.624367 1637.780067 2151.888600 2667.149433 642.575027 524.639100 1714.352633 2189.275633 471.228190 1113.507067 1897.421133 1879.989400 5879.698667 315.511950 330.304503 1747.672400 943.612587 797.811967 2272.925900 6072.304467 951.663767 2612.369167 1669.684500 8923.779000 3173.168867 485.919740 1602.313867 843.184077 2057.959667 852.195093 689.099900 431.554187 616.738323 5835.765433 5035.098633 1493.285300 1037.867433 1292.762680 1317.997733 1569.749200

164.771100 293.138800 326.276403 651.573783 955.143947 1238.151320 235.891187 165.631247 581.792457 636.236283 205.400327 534.792913 619.864320 828.720047 1347.244667 142.994397 135.747653 595.683857 301.209090 373.836020 990.235620 2130.935833 344.585060 1136.369833 474.393757 2389.688900 753.208870 179.684457 779.781780 383.486793 732.631147 278.491910 281.948530 140.287840 199.346397 1487.953967 1220.405833 333.457997 227.777103 503.847713 594.676253 340.149943

NOTUM PRKCH ANKRD15 DUSP8 ITGAX CCL14 N/A FGFR2 ERG CYP27A1 ECHDC2 GGTLA4 WASF2 TACC1 PTGDS LOC153222 C3orf46 RCC2 TRIM25 PCDH12 RGS2 C2 MEIS1 GGA1 MAOA CXCL16 CCL14 CARD8 PTP4A1 TMEM37 PPM2C ZNF533 TCF7 ACSL1 NGFR MMP19 GPX3 TNS1 TRPV2 PTPRM HDAC5 LRRC32

224.555397 1492.972933 118.534972 271.866263 293.421040 1766.541133 501.611513 1089.235533 203.616417 460.302687 194.275993 339.772187 153.642913 289.315290 4533.570667 845.065053 186.414117 701.626717 1174.431833 1104.511400 330.850987 504.418553 1736.665767 405.367510 1745.855567 155.003030 242.460423 147.826973 1196.567180 464.024080 294.638993 198.198607 692.568683 1136.540300 1241.413247 358.730123 197.328213 132.241900 426.832673 590.439650 189.307330 194.353497

967.871390 3437.615533 566.168417 603.739583 893.950807 3849.013300 1176.604733 2603.493467 761.910453 1247.699000 453.762593 776.632430 506.380843 674.764880 10234.246667 2070.829233 558.417293 1592.061033 2598.291733 3220.393800 760.356583 1132.116290 3673.609700 1658.331600 4356.630967 316.183590 729.528287 356.973493 3765.944167 2230.575567 804.169187 400.000450 1562.924233 3103.124167 3225.916567 1066.334080 615.306827 338.762973 1334.439517 1769.534700 405.628513 450.837810

DSG2 RPS4Y1 MYBL1 SEMA7A MCM10 PTTG1 VRK1 HMG4L CDCA2 ORM1 ZIC1 RICS RHOV SCML1 C20orf52 S100A2 SAA1 IKBKE SUB1 CCT6A DNAJC10 EFNA4 RPL36AL CDC2 NPM1 NUFIP2 CSE1L FUT3 HIST1H2BK CSE1L LARS ZNF146 HSPA4 N/A ISLR SR140 CARD11 ATAD2 RSN ZNF185 SENP1 ABL2

2715.298100 1989.865100 673.313467 5492.830233 1298.871667 3952.234633 924.287487 1676.856700 1639.998133 713.740717 1188.908983 404.774947 1223.953967 258.992923 1751.023800 331.285303 1101.548467 500.551020 1132.672533 1332.305967 2236.649433 805.795300 2904.519267 622.789820 533.352460 797.367467 1061.787950 2529.355000 1494.442833 8254.569000 2010.218500 3605.886400 654.671487 2170.778833 948.093300 1918.216433 522.019740 469.721947 417.809633 1979.239067 429.403110 395.358510

1091.457533 838.663067 232.967220 2694.463000 488.650237 1085.400800 310.653490 739.061103 444.960817 336.613997 473.167267 176.807613 370.132293 113.662430 439.187653 151.687770 366.935523 240.719850 485.956903 443.394173 534.621607 292.710730 1217.401233 268.021103 247.320780 336.661503 398.753007 1206.139933 672.310013 2857.638667 933.929280 1078.086133 223.390313 619.530933 304.354213 914.535000 190.893183 150.122240 201.435970 906.692233 195.485990 175.804463

ARHGAP4 TBX2 SSFA2 MRPL10 EFNB1 THBD ARHGAP30 RASL12 RCC2 ZNF179 MMP15 NINJ2 CFD FLJ43315 HP FLJ10847 ANPEP CYP4V2 CST5 ABCA3 PRF1 ANKRD44 CEBPA CD40 APOC2 CYBRD1 KIF17 LAMA4 NR4A2 RHOB BTN3A2 HR N/A POLR2A GIT1 CYP2B6 IL1R1 IL1R1 GPR4 IL7R CASP8 FBXO33

497.513767 252.560950 511.345143 1485.584500 713.404840 215.688437 2797.947300 277.085243 169.618037 127.217543 2845.605800 1690.804100 367.062473 250.552413 1793.029400 1966.265800 402.441060 1373.481900 131.247602 269.668163 275.661243 5025.001767 11530.699333 438.576947 441.814580 663.067477 6133.234000 257.284480 175.093583 136.353537 354.901960 150.379263 1062.932743 178.128477 565.297633 532.868247 352.776100 368.623477 1413.234633 897.478450 599.183880 1029.798700

1663.397533 679.488690 1853.804633 3166.404133 2289.090200 673.464983 9205.899000 602.152597 432.398010 299.285630 6533.100567 3754.213233 910.417477 814.538347 3703.867767 4253.656633 954.187600 4540.289700 302.172997 653.914300 568.861273 14421.064000 32292.072333 1948.446467 1453.764700 1879.970800 18707.561000 767.997587 540.983213 416.447423 746.881533 387.814703 2867.769133 675.472163 2744.100667 1202.305633 923.645790 999.450867 3694.200100 3105.688867 1852.056667 3376.560867

WDR62 CRYZ SNX6 C10orf7 KIAA0391 N/A AP1S1 C14orf80 KNS2 N/A CCT5 EIF2S2 N/A BCAT1 FLNB CEACAM3 CELP CEACAM6 ZP3 BCHE BCKDHB COL1A2 COL3A1 COL7A1 CYP1B1 MSH6 LAMB3 MET MSH2 MYO7A PAX6 SAA1 TPM1 OXCT1 IGFBP3 ORM1 IGF2 ADORA1 AMY2A CHRNB1 CRAT PLAT

1059.808887 1337.543267 858.675133 2870.405500 7809.722333 550.177930 700.966800 1440.028533 666.010000 3116.568833 581.531660 1912.711867 1790.372133 7187.820667 1416.855133 1307.047267 29165.716333 5172.341467 1605.179433 3296.161433 19227.620333 1910.743433 6072.658333 886.420420 747.773720 16070.667000 3612.976533 972.125567 2385.977200 526.662887 1053.799133 610.157850 561.434787 6708.123867 10793.102000 1252.679667 654.068543 1105.098800 337.690090 513.810647 607.763563

470.263443 649.710377 356.328497 1371.394933 3453.875867 268.506120 314.056670 354.517313 191.156047 845.604047 203.209710 438.730520 535.705113 2472.476233 685.476500 526.430093 13182.636333 1905.828300 369.452870 1016.622550 5764.804167 708.589843 2425.428733 426.590593 343.547433 5111.808767 1802.327967 281.508607 1015.870267 223.945640 524.662123 186.936572 188.225023 2705.493533 4317.629000 552.091597 173.835007 299.430547 156.776633 204.805233 287.495780

LOC49412

7 FPRL2 TPSD1 RCC1 HBD EFNB2 SLC7A1 CAV3 KLF9 CCL18 GPA33 LRRC32 HP MGP IGLC1 SNRK HLA-DRA KLF2 GJA4 N/A HLA-DQA1 EGR1 TPSAB1 IL1R1 PNRC2 CAV1 KHSRP N/A AK2 ZMYND15 FLJ41131 PAG1 IGKC MGP TXNIP RARG NCKAP1L BCL2L10 ZNF664 LOC124216 RNF152

1252.503400 979.271053 2083.525700 1890.101000 118.399991 235.716163 239.517203 155.241883 153.759518 235.074927 296.509793 165.438852 4193.239533 120.496512 955.996223 507.230793 1461.266167 459.336897 127.001217 2239.851000

1240.599267 214.355390 198.071890 1697.033567 171.058980 2268.477700 686.501600 211.359967 610.762593 205.643290

8550.065667 434.930533 244.023593 1882.494433 1237.258833 1643.124000 1018.007347 699.518400 595.178373

315.976963 5443.848000

4744.210200 1968.077400 4320.192733 3909.683967 316.239357 868.653540 839.619267 374.516717 458.535347 645.887860 618.934680 525.269177 8962.753533 275.557913 2658.582467 1420.933567 4064.934100 1126.905533 438.409273 4510.785400

4440.828600 965.796010 404.704710 3662.962700 472.081200 4668.053233 1867.028333 941.340967 2537.068133 417.153497 17821.349667 1004.612400 806.285163 4154.390967 2742.531367 3705.903100 3354.595100 1548.137233 1772.440600

668.941100 11625.426500

PLAT PPIC RPL36AL TBC1D3C DEFB108B GATA3 SMC4L1 ATP5S OR2T33 OR4M2 OR4N4 OR4M1 SDC1 GOSR1 ALG8 KLHL5 C11orf43 IFRD1 NLF2 RPS4Y1

AMY1B LOC493869 TMEM64 THAP7 LOC401052 CKAP5 CCT6A HS3ST6 PHF19 HERC6 BCAM LOC541469 C1orf192 LOC391356 STAP2 OCIAD2 MGAT2 TAF9 CCDC58

P4HA1 HSP90AA1

51基因芯片_基因芯片

1642.882733 645.944870 2680.627500 652.510267 1170.822480 540.111070 532.044940 2549.025767 2048.772900 1351.097567 738.146190 406.283833 746.762427 824.047317 1010.236800 670.557770 1111.524733 434.111033 387.421600 462.366500 702.435033 1945.579167 3930.026767 1865.010433 5658.486167 1756.639267 676.424580 316.529647 854.867803 562.972353 564.407467 429.041467 583.917100 1557.771500 1449.274967 28222.270000 310.379110 847.301033 9097.637167 287.192060 463.591040 1613.245967

691.357000 303.941367 814.761790 194.686118 471.284223 156.244423 178.797880 790.601097 894.573513 288.995940 306.276217 169.997085 310.935383 228.542818 250.818113 281.662747 304.490437 179.573720 155.485760 192.823003 214.350930 574.859213 1671.909233 642.164943 2726.230400 783.337710 229.018133 136.000528 339.535907 264.297063 265.402103 170.741800 213.134220 745.725643 412.514117 13741.909333 137.253890 327.875943 3394.436533 140.576747 187.032072 516.226557

KLF10 1217.338233 2784.358267 TPM1 ING4 1064.466187 2244.552433 TPM1 CCL18 369.221657 927.582347 FANCD2 HS6ST1 492.671100 1183.203667 APPBP1 ITGAM 3281.118767 6929.586033 S100A13 WNT10B 397.701727 1154.524933 FBLIM1 WNT10B 655.368500 1825.396233 FBLIM1 PPM2C 198.259810 418.610400 TAF5L JAG1 4425.865767 9392.986667 VEGF AMICA1 178.869933 494.213727 SPAG16 HAND2 636.044313 1450.325333 UGCGL1 LOC54103 929.942220 2662.982700 AP2B1 PHACTR1 663.817090 1502.829933 KIAA0020 AXIN2 463.113090 1058.177067 GPR126 RCSD1 224.066713 476.253053 LOC440888 PTGIS 757.811547 1739.123067 RRM1 CAV3 615.443843 1472.768867 SAR1B CHIT1 210.087357 619.780043

CSTF3 1520.370033 3219.842367

CSTF1

LHX6 1534.378200 3080.593867 SPECC1 FGF9 329.837427 787.845313 ZNHIT3 CCL4 836.391080 2212.412733 CEP250 N/A 1611.069800 4364.566067 NPM1 GYPC 586.876893 1722.510967 FAM7A2 PKIG 459.927083 1398.400533 ADK N/A 3011.268333 6918.329800 AEBP1 PLEKHG6 150.885093 622.451907 ANGPT2 EGR3 220.734430 617.230000 CA12 DOCK2 1741.868167 3732.183667 CALU CD163L1 208.479257 466.007830 CCNH FLJ44186 125.008293 461.931167 CCNT1 MPEG1 279.424320 645.386100 ENTPD5 C17orf58 283.610597 569.660767 CDC5L EFNB2 296.397973 974.185233 CDKN2C GYPC 1408.627433 3539.641467 AP1S1 HLA-DRB3 877.371017 2659.471567 CLDN3 MLLT7 657.607927 3167.407933 CSE1L ETV1 243.078330 501.886107 CSTF3 LAPTM5 1291.030913 2677.994633 DKC1 DLL4 325.000113 705.656600 DTNA GPA33 1165.936067 3089.591767 FLNC CCL4

164.677383

403.667740

GAGE6

2361.349467 9658.297100 2766.193100 1195.474400 31331.764333 613.319823 733.077633 2227.327200 339.328087 2082.934267 2208.865233 912.278033 470.468017 324.841490 6241.540867 1142.253420 1112.084467 9008.074333 1490.385533 8380.069100 635.101813 770.310757 490.950957 763.804920 1231.475467 2840.473367 628.921587 1138.785100 732.635543 565.243917 961.387800 1163.701533 1772.555233 271.794793 3212.665200 2199.650533 1689.658767 336.193817 379.226713 2495.605000 928.708933

539.265253 2461.315500 1333.758467 594.859703 14934.182333 294.811723 256.808123 864.688603 122.915905 973.380983 994.120100 345.767987 166.589090 129.637172 2877.677933 503.423117 416.050840 4304.653467 481.595823 3986.582567 181.774493 190.166485 206.029860 306.513510 329.048573 1131.141133 238.230142 482.410473 235.504277 155.479262 408.423143 559.103217 640.935923 125.101195 1480.994633 757.349747 537.194393 164.214290 172.099483 856.553863 381.414397

CCL3L1 N/A BMP8B TJP3 HLA-DRB1 ZNF518 BTK HSPB2 TMC8 PIGR USP22 C10orf76 LOC91461 EPN3 SULT1A1 CCDC57 RHBDD1 UNC84B KIAA0040 UNC84B SESN1 CGNL1 NEK9 PAG1 CDGAP CAT ELMO1 TTC7A DAPK2 N/A MBTPS1 GSTM2 WDR23 PDE6G NFIX CD34 TENC1 C1orf171 RAB2B LOC388272

KIAA0703

261.047453 2306.515467 425.969313 432.385623 352.482033 798.725553 501.025300 123.112593 493.651520 9323.987667 275.045473 453.111300 395.565360 252.025537 763.407657 683.355687 716.265067 162.994510 6903.546467 1590.381087 3064.056967 136.891480 326.722590 903.327387 661.315933 321.787087 331.647580 2553.472733 1251.844067 197.089120 625.576307 230.069630 894.554977 375.480873 307.345323 1345.621467 2880.255933 114.456777 2085.438200 1604.886333 6348.270133

657.335783 4645.286633 961.754890 1293.034600 1387.395120 2543.511333 1433.194483 257.794547 1032.953213 21965.755667 659.150753 1024.252467 1603.424667 560.750157 1697.811167 1421.388800 2702.299233 510.013450 13858.748333 3321.006100 6690.645200 472.037647 1592.439967 4152.042733 2315.765867 1356.263900 788.036027 5419.098067 4339.563633 904.138567 1417.691100 959.615677 1985.230133 966.983037 708.050277 3777.612533 14255.806000 257.079520 4318.262700 3319.125833 13294.677000

GPR39 HDAC2 HIF1A ATP5J CBR1 CDA CDC2 CDH2 CENPA CRABP2 CRYZ CTSE DSG2 GABPB2 GFPT1 HSPA4 TNC INHBB ITGB4BP KIFC1 LDHB LOX LOXL2 LTF MMP1 MMP7 MNAT1 MX2 CEACAM6 NGFB NRD1 NT5E NUP88 ORC5L PAWR PITX1 PLAU PSG7 PSMA3 PSMA4 PSMA6

610.676683 6177.674733 650.818087 2993.327867 5370.713433 4563.762867 1354.136400 9688.333433 529.117757 939.985710 2045.502500 1057.795380 2104.282133 412.724567 471.063017 1108.287633 3166.658867 900.177573 801.825833 4727.409900 1941.056833 4841.093633 1909.217333 4536.184967 14218.144667 3793.493833 12458.235667 958.800467 20608.871000 3258.969900 1348.506200 369.224150 4556.754400 878.070687 7853.963633 5537.755633 5231.322167 1558.969733 2630.525467 297.584813

247.676980 2698.460267 256.947973 1262.583033 1501.886633 2228.874567 573.739657 2578.615767 240.784583 390.906293 1014.188543 296.495830 491.794250 160.798347 165.264987 510.235983 1488.880733 350.497690 251.396093 2236.685833 449.036097 1885.354467 679.506867 1729.950233 6545.165667 942.225267 4370.486133 281.494340 7193.024967 734.258740 637.133203 171.334043 2162.915500 303.340177 2575.429200 2484.493033 2279.939800 613.262870 537.108107 110.865671

APOBEC3D EIF3S6IP ABHD6 FDXR FCGR3A SERPING1 EPOR PTGDS 182.261737 C10orf128 PARVG N/A SPI1 SLC35A1 STARD9 IL16 BAZ2A KIAA0703 TYRO3 TRIOBP TGFBR3 N/A NFIX N/A HLA-DQB1 CLU HLA-DPB1 CSF3R HLA-DRA FLI1 ACVRL1 SGCA ASS BTK SERPING1 C2 C3 CA2 CPOX DPYD

171.294673 777.127567 PTHLH 456.512273 916.533933 PTPN3 1058.898600 2282.462067 PVRL1 444.101297 1311.975720 RBBP8 150.631797 330.207350 RECQL 238.835827

562.833403 ABCE1 534.265147 1996.409933 UAP1 322.344607 1136.186400 SPAG4 525.773107 SPRR1B

1314.850867 3085.886200 SRP54 466.766820 2064.427167 STC1 163.187033 349.657687 RPS6KB1 234.046730 626.634813 TAF2 870.904600 2040.121433 TAF9 884.895947 1905.089200 TBCE 524.160767 1323.198783 TCEA2 210.403777 1034.401057 TFAP2C 5755.190733 14205.177667 TIMP1 343.886210 881.542633 TSPAN6 1012.245567 3612.955167 TXNRD1 416.762667 1033.866050 UBE2E1 233.088403 588.349283 UBE2V2 2027.660367 4499.435233 SCGB1A1 857.353537 1899.232067 UGCG 1818.854900 7797.138000 UPP1 790.477303 1602.685333 XPO1 1421.077800 2864.374533 YY1 365.707937 865.990200 ZNF131 519.416000 1392.761267 DEK 1230.572200 3684.315533 TAF15 183.725387 485.938623 CDC7 615.027647 1242.186167 HIST1H2AC 183.777043 423.972190 HIST2H2BE 225.350600 474.705250 TPST1 440.277730

1330.197367 SMARCA5 437.409963 1049.183967 CNTNAP1 472.951387 1098.958067 CHST1 894.761007 2406.271667 ABCC3 762.350843 1770.196467 CTNNAL1 306.408513

905.203087

KYNU

1021.903567 2325.158767 7053.020000 2694.792800 584.166307 3727.330633 1103.397300 1706.033733 497.839320 1774.353767 3249.556333 1791.061700 1038.099667 880.702600 11512.016333 497.026700 587.232720 5079.885333 1719.523667 646.178920 832.907640 990.607763 5836.360533 23154.931000 2851.621233 10418.993000 6399.359433 20257.282333 2823.751133 1953.549467 4513.194933 5692.481567 803.305923 5216.851400 5492.081233 1119.888700 3409.048200 544.218747 2359.283067 813.839167 378.901130 13319.119333 2232.233367

377.683423 530.949367 2762.135000 1263.525300 275.117217 977.520317 372.637320 762.908590 201.191807 425.235497 1069.654687 768.780163 467.063580 232.895663 3108.909267 214.417973 222.295043 2510.848867 374.368673 238.081560 333.673633 337.244883 1192.351800 7921.220667 849.110087 3777.147800 2239.840100 8498.029767 718.867953 528.088323 1202.493200 1532.442300 180.156100 1662.050500 2374.042833 394.839313 1468.912567 242.607350 879.544287 351.734680 145.313663 6223.677067 601.431887

F13A1 FUT1 GSN IL2RG KIT LAMA3 LIPA LYZ PTCH NCF1 SERPINA1 PMP22 PEX5 THBD TIMP3 TNNT2 WAS AQP1 CYBB GP1BB IFNGR1 NCF2 PECAM1 C1QB ELN FBP1 HBD SFTPB VWF CD53 FCGR3A FCGR3B IL1B SLC11A1 IGFBP2 CD44 CD4 NCF4 RFX2 BCL2 ADORA3 ALDH2 ALOX5

1604.731367 784.876957 1800.201700 462.768930 595.223000 389.074633 223.477330 279.901880 924.534650 696.848920 567.379690

2560.368267 151.266473 703.312593 227.026930 290.375100 2921.556733 147.796385 175.893707 2446.331033 216.327330 168.655632 309.701360 428.019527 397.816977 888.595367 732.851713 736.829833 794.380117 233.158077 1962.845300 523.950350 213.635953 216.553583 164.753893 1294.886200 386.666917 4733.352667 215.396853 727.318833 199.099800 345.406260 360.119340

4519.234067 1744.965800 5330.016333 984.294800 1620.224567 936.992800 845.932987 866.238617 1952.840767 2104.931167 1601.752133 7057.979067 598.421100 1518.084133 632.778017 673.333377 6081.735033 388.107573 629.788450 6619.234767 855.228290 353.242140 975.389467 1339.975267 1044.684967 2850.156967 1619.340933 1748.675633 2637.725533 635.833033 4575.427400 1233.335700 707.916467 494.303303 518.746177 3718.943233 894.438403 13343.957333 491.516327 2128.027400 465.868887 1320.647200 1003.644933

BNIP3 LOC552891 TRIP13 SEMA4F FADS2 BAG2 ASPH BUB1 CETN2 COL12A1 CSPG2 ECM1 FAP FUT8 GNG4 KIF5B FANCG EIF1AY GCNT3 TJP2 HOMER2 RASAL2 KIF23 PTGES DDX1 MMP14 THOC1 ROD1 CDKN3 EYA2 HIST1H1C XRCC3 APBA2 BCAT1 INSL3 ISLR KNS2 KRT7 KRT16 LOXL1 ATP8B1 REST TAF7

579.008420 1467.854433 988.832400 2677.807400 1478.964133 10034.418000 2102.323700 535.737113 1590.841600 5605.307367 9304.328467 825.383457 449.635867 12103.832000 431.232350 1020.412190 943.455400 1113.822000 1871.799367 657.802660 454.991087 589.950487 4166.303133 1296.685300 1658.705700 5562.068000 977.875507 686.305600 7921.481400 545.503563 1912.379967 870.446467 1743.155500 532.388477 755.104633 811.338610 783.713517 479.058510 1867.949500 3174.992767

125.386788 446.430257 268.023897 979.632587 581.795553 3552.005900 584.419853 254.869803 771.615050 2111.675667 2632.307833 244.368860 162.168887 3447.328333 150.985932 458.359820 274.865703 390.291757 906.882020 289.473170 218.231250 227.662847 1401.170767 444.318740 791.744767 1693.723267 275.613447 330.829467 2750.876500 172.754967 873.285817 355.540590 742.882350 184.863683 237.130087 365.938993 325.016017 189.200807 865.078447 688.070980

CYP24A1 698.908320 1980.890900 CYP27A1 138.326193 398.238887 ACE 691.710453 2032.887200 FOLR2 312.047960 899.665487 GSTM2 3248.440233 8643.455500 GSTP1 385.360420 1020.695620 ICAM2 2477.813800 5366.040033 IL1R1 234.895860 785.431733 ITGAX 563.741600 1357.234600 LTA4H 617.633950 1277.521100 MGP 646.836880 1371.622833 SERPINF2 1202.658400 2523.449167 PTAFR 358.631917 1330.264933 PTGDS 199.218777 530.426627 SLC2A9 799.568700 3181.797367 CYB5R2 772.319033 1924.381167 CCL4L1 640.112800 1660.541667 CCL3L3 917.249453 2121.398133 bA9F11.1 4202.256233 8510.093000 DDI1 437.595393 1523.538667 VCX-C 1560.911700

4004.681400

1251.887833 2530.848733

PAIP1

SERPINA1 611.533667

2513.011533 C9orf58 718.253847 1689.003400 FCGR2B 234.943197 543.630143 RAB11FIP1 172.967347 368.775933 ABLIM1 181.874280 519.522767 C11orf49 655.110517 1827.190600 GPNMB 304.042437 1365.284100 SF3A1 601.623403 2029.506900 FCGR2C 1080.152900 2668.738133 REG3G 649.216733 1449.886233 CTDSPL 407.475513 886.567877 EML1 247.361397 1087.957467 KIAA1754L 5984.024767 12007.176000 SH3D19 170.825577 612.992633 AFMID 195.114280 491.807873 DSTN 483.522740 1432.506633 LOC348180 213.756607 439.767170 KLK6

815.735050

1908.530700

TSPAN1 CEBPZ MPHOSPH10 GDF11 TIMM17B NET1 FMNL1 MAP1B MCM6 UCHL3 PNMA1 PCNT WFDC2 PCM1 THY1 SHFM1 SLC30A9 CREB3 CIB1 AGR2 TXNRD2 SPAG5 TRIM16 GNAI3 RASA2 CGREF1 TPBG TUSC3 RAB32 METAP2 CCDC85B HOXC9 SLC5A3 UBE2C TOPBP1 FGFR1OP TRIO ZNF146 ZNF185

51基因芯片_基因芯片

1886.548267 2220.989133 4080.148233 1650.670633 985.098067 481.140523 1449.734583 4990.787333 934.657450 604.929543 2180.010867 733.407153 2849.658467 3329.262067 796.366943 701.808127 7970.224000 443.002643 746.446013 2103.376633 2541.966100 17669.270667 1146.428000 1456.777733 1059.670580 2124.171867 388.187973 2349.174700 1562.319267 649.488113 3449.783200 1481.505800 752.636030 2457.744667 1897.521900 5539.215467 1769.946267 2098.429233 1867.125200

661.259180 828.965367 2015.544300 806.333640 318.483793 223.659400 291.285677 1640.583367 344.545657 244.547897 810.243457 238.963217 1090.953477 882.980347 180.487570 145.469667 3501.774900 211.462387 308.258333 948.643150 773.337647 6283.716800 411.092030 483.584727 297.530877 456.177600 181.477503 766.155550 752.541467 272.471053 1672.733267 363.350690 322.860367 900.270000 645.070233 1667.285967 450.910797 892.271523 754.205510

LOC40162

2 RLTPR HSP90AB2P DDR2 CRMP1 LAT CKMT1A TSC22D3 COP1 KIAA1026 KIAA1026 KIAA1026 SH3BP5 ZBTB16 Gcom1 Gcom1 LOC401623 GOLGA8B SBK1 TGFBR2 CD34 CD74 TMEM16F C1orf138 ARHGAP30 RSPO4 MGC26718 ATF3 SPN IFIT3 YIF1B LOC352909 CPT1A SLC11A1 KLF10 MMP19 NPNT PRKCZ LY9

1433.170633 609.345030

2297.974933 338.733023 169.214847 347.596363 1088.493717 338.577807 841.536400 1017.120200 282.834760 281.500720 868.460783 3002.880267 599.135923 2074.036667

158.764173 917.892957

1847.341733 381.805410 931.431363 170.493423 285.619540 284.987473

2981.655500 1846.326733 2549.325600

223.584733 1149.188667 788.590217 511.854660

850.228763 161.171259 401.600207 439.390690 553.815403 365.733490 1429.055233 208.412953

3011.228033 1261.004500

4654.998333 858.057567 522.798737 1153.691047 2365.643567 825.010033 2518.628333 2575.064133 646.106207 658.536620 2105.932900 6305.703467 2362.433400 4297.351700

401.992753 2135.313900 4041.598100 846.786050 1982.432100 506.252280 730.013797 662.138143

6579.767367 4005.252333 6570.887867 1007.968387 2777.035433 1890.273733 1211.812833

1952.643500 442.418590 1952.161767 1062.769233 1570.555500 804.428730 3413.074367 471.945130

SUPT16H DUSP12

NRM CBX3 BRCA1 PHLDA1 COMMD3 ANGPTL2 BACE2 TPX2 CHORDC1 FBXO5 LTB4DH SEPHS1 LDOC1 GTPBP4

PRND TBL2 CLCF1 CARKL NME7 FST UBQLN1 BAZ1A

FER1L3 NUDT5 ORMDL2 RAGE TMEM5 RUSC1 FJX1

GNL3 ZBTB32 LYPD3 LSM1 SRPX2 SENP1 RHOD MTFR1

723.945850 16246.046000 5808.634300 1504.143767 735.855980 3566.091033 707.675883 2786.335267 1124.042957 600.415310 546.090320 779.733580 1034.392067 1404.852167 3255.009400 5789.423833 2390.771833 528.626823 1712.083767 1653.054667 627.852347 2682.385800 942.361933 1030.366483 863.466567 2802.687900 1375.410700 997.753413 1105.473733 664.949580 1044.327433 637.423757 1246.605267 5802.252733 2809.121300 1059.564053 4759.010567 4597.545433 1243.962967 1748.673733 358.274780 706.652280 1889.347167

290.412353 4653.201433 2572.519700 387.137940 240.619767 1550.330067 249.984287 980.120113 239.129998 216.426830 184.638923 364.642313 385.543987 504.514880 1153.006067 1937.998000 1032.625617 195.023153 383.882660 508.816920 281.571377 807.633293 311.097197 296.590360 416.922990 1115.356467 678.729703 304.515847 423.524800 295.166583 463.170877 170.304560 579.285453 1701.990833 1032.512287 360.839700 1893.259733 1238.720147 452.995047 695.305833 144.031440 313.413057 720.804650

PDE4B N/A SCNN1A ICAM4 SRG C20orf191 ELMO1 C1orf38 NOSTRIN FLJ16423 FLJ41484 FLJ36445 GPR30 SIRPA CD37 CD68 SULT1A1 TBXAS1 ABCA3 ACACB ANPEP AOX1 BMP2 CASP1 CHKA CIRBP CTSW DAB2 DUSP9 EEF1A1 EN2 FOXF2 HSPB2 AHNAK ARHGAP4 C5AR1 CAT CAV1 CCND2 CCND3 CD1C CD33 CD34

895.665613 283.089777 1072.622733 1460.171767 441.624203 408.177700 284.880077 287.235140 246.750840 320.002957 1207.924067 257.869967 490.051800 2515.827233 542.693680 519.106430 323.934247 981.614183 270.665807 679.862350 222.804367 575.013913 485.835370 793.931443 1723.891200 1045.589653 207.557273 1255.601843 314.183817 729.724917 3863.295300 312.913980 500.737520 229.657583 1251.738067 452.149090 481.945657 196.890443 384.356580 442.865893 305.394420 298.277017 495.434817

2035.241300 620.364187 2209.331533 4095.024600 1560.123200 932.650687 1115.827900 987.792323 520.565023 1270.852267 2557.322167 604.270797 1146.105100 6285.218700 1136.827167 1707.526613 755.466800 3832.880700 895.190513 2028.925300 565.437333 1270.707800 1317.302667 2817.248267 3978.353300 2143.708933 756.848867 2600.415367 760.453347 1677.114000 11715.788500 1336.477767 1571.157267 890.837417 3286.097167 1071.466100 964.613767 479.959513 978.023000 1439.647900 852.136053 769.211217 1247.120933

KIAA0391 KIAA0586 BMS1L SCRN1 MELK RBM34 PPFIA4 KIAA0802 TRIM37 MXRA5 RIS1 NOL11 C1orf48 C18orf10 RAI14 GPATC4 GPX7 G0S2 GMNN NOP5/NOP58 SNX7 ATP6V1D SCCPDH COQ4 C14orf166 MRPS33 SCFD1 CPSF3 HSPA14 GPR89A PRODH CENPF NIN NUSAP1 ACP6 HSPC148 GTSE1 SCAND1 BCCIP FKBP11 RSRC1 DACT1 C21orf6

1323.224400 789.783573 590.158833 1020.753733 1663.268733 12151.526000 2300.229933 1559.506400 3019.930433 478.873603 3106.809133 1198.374667 597.453563 3562.235867 1268.457967 1645.339933 3815.774500 1797.340333 544.707570 2626.806633 1616.193800 16042.623000 9966.646333 14544.820000 13165.842333 1075.071267 451.830340 5242.556333 5300.043667 2492.180733 1091.936033 1734.621167 1077.759857 926.423073 3383.420267 858.263713 1336.032700 3129.867000 1211.123633

611.437970 235.015773 278.960730 384.199707 478.908880 4507.585400 580.811113 578.074317 1088.858423 157.082632 1010.385397 388.401113 173.722717 860.889793 387.285007 342.607807 1005.895720 611.090060 128.387777 1174.716267 652.395733 5025.558000 3718.253233 5356.135333 2977.627600 468.255187 152.702243 2236.132100 2323.651400 1131.603600 389.793407 585.315333 514.815787 413.871733 1579.845733 278.236187 531.176890 1325.849800 559.228737

CLU COL9A3 SLC31A2 COX7A1 CRYAB DCN CFD EDN1 EGR1 EYA3 FBLN1 FCN1 FGF9 FGFR4 FPR1 GJA4 GPX3 GYPC HCK HLA-DMB HLA-DOA HLA-DQA1 HLA-DQB1 HLA-DRB5 NR4A1 HOXA4 IRF8 ID1 ID2 IRF1 IRF5 ITGAL ITPKB JUN JUNB KCNJ15 LAIR1 LCP1 LRP3

140.172098 338.808807 387.599683 448.528977 1009.805900 346.011297 752.358380 222.004827 1205.233233 464.617080 1488.497600 182.864243 504.774493 473.212383 302.938973 367.989847 461.795177 480.582107 206.425690 311.932197

282.140613 287.157407 343.208297 572.585910 237.667227 234.359707 663.893673 416.488187 182.678323 873.234530 672.878580 198.461517 622.358977 1132.096733 210.455887 161.191353 1130.994267 300.864160 1586.630433

408.572860 867.632423 1239.224733 906.276513 2047.994300 1237.391733 1514.096833 800.277370 2556.599400 1337.682933 3339.037433 505.758007 1017.333953 1363.463667 772.038633 1388.195367 1420.777067 1000.909967 420.892363 937.365517 576.258240 1414.407667 686.920833 1183.333633 736.581947 805.893017 2338.296967 1013.394077 480.375257 1833.950900 2406.286733 609.426283 2196.047567 2504.992633 483.024963 339.252117 3885.098133 649.328757 4237.571867

HOXC13 CALML5 MRPL39 BTBD5 BCAS3 BSPRY EPS8L1 FLJ20272 NSUN2 CCDC99 LYAR FLJ20516 INTS8 PAK1IP1 C14orf10 PEX26 NOL8 GPATC1 NODAL CEP27 DARS2 CEP55 C14orf104 MRPS10 SBNO1 KIAA1794 LRRC20 KIRREL STATIP1 PRR11 BXDC2 PI4K2B STRBP DKFZp762E1312 CAND1 KCNQ1DN SMOX MRPL50 POLE4

979.350813 1073.295767 612.968620 2031.858600 8860.738000 918.858653 345.605930 1081.809900 4875.948400 1406.102167 1835.750933 1399.089600 700.036530 6712.488500 2598.309433 1196.152433 1326.541753 3654.356933 1825.029033 594.112957 21989.350333 1025.709190 3267.649867 1429.807033 1508.810267 3438.504567 937.879147 1080.554500 8532.301100 3933.920733 3222.030733 1242.189367 838.941833 28685.999667 1149.277067 248.206017 1225.903000 756.329753 1725.788033 2250.679533 1322.339500 1871.938333 847.370033

313.177720 487.824503 275.178977 934.421267 3775.659967 400.431723 166.496767 350.446453 1867.012267 565.557577 841.457113 531.717800 199.795120 2831.721367 989.961413 423.169513 583.450300 765.904047 781.120223 211.118552 10304.013333 314.592470 1232.805800 615.562753 455.379620 1080.096433 415.016080 372.696877 3446.652633 1840.564967 932.221133 374.891377 328.845373 13048.469500 278.480330 120.494658 307.564553 215.494953 525.293633 478.149900 351.825510 923.340533 375.558290

MEIS1 MMP15 MNDA MSI1 MYC PPP1R12B NDN PALM PCDH1 PIK3C2B PLCG2 PREP PTN PTPN6 PTPRC PTPRM PTPRS RBP1 RNASE4 ROS1 S100A4 CCL4 CCL18 CCL21 CX3CL1 SELPLG SKI SLIT3 SLPI SMARCD2 SNCG SNX1 SPI1 SPTBN1 STAC XCL2 TAL1 TCF7 TGFBR3 CLDN5 TNNC1 TNNT1 TPSAB1

498.782983 2809.988000 141.624333 182.886713 1718.254067 227.367810 3752.349167 437.848300 614.234967 231.500573 207.975953 2155.954333 704.352800 390.379763 397.850843 221.325497 313.299907 328.242227 1344.092433 1270.989300 983.507390 1672.880733 442.591180 206.056123 263.284797 411.247880 1549.801367 125.367522 1329.661033 401.930167

150.179560 2357.304200 1782.008500 586.719567 438.805500 714.317510 698.024210 839.371233 636.517407 906.933357 873.916533 730.661667 160.292593

1461.555833 5665.322433 518.653807 396.420233 6739.903867 720.071933 11142.826000 1202.007033 1687.163500 470.440807 566.864357 5781.132767 1444.975167 1289.944033 941.891050 906.845587 868.483927 1410.813590 3091.203067 2843.834233 2289.034900 3495.575000 979.932917 452.830567 540.684953 991.620833 4894.994733 424.563310 4892.058467 843.030557 445.804500 7771.453333 3695.497433 1346.971967 887.309233 1601.643067 1815.846133 2117.747067 1363.346800 2375.634900 2602.372767 1628.961567 408.175623

GPR137 ASPH LXN SHD SEMA4B PRDM8 MGST1 NUP107 MRPL47 TMEM63C GALNT1 RCN3 DECR2 KIAA1143 GM632 NLN TAOK1 4-Mar ZNF687 DDX55 KIAA1641 AMY2B UNG2 CROT RAP1GDS1 OVOL2 SNX6 ALOXE3 SPHK1 EGLN1 ICA1 ECM1 NUCKS1 C1orf176 SNURF SNRPN SNRPN ZSWIM4 LRRC61 L1CAM C7orf24 MGC4172 KREMEN2

19899.761000 609.382917 6664.585800 810.076533 554.055267 4547.733333 964.903347 990.228933 532.051083 4349.577200 718.229317 2935.733400 9775.204000 853.351287 936.953433 490.939590 2235.757333 653.634800 870.433520 1491.312700 3362.142200 2159.049400 790.696370 2885.323500 913.688247 8502.920800 2481.144433 1205.459733 422.249980 1127.148600 2270.906267 2214.966167 1635.569167 4278.052367 6532.170567 4385.805900 2195.486300 742.948220 675.156530 3953.323633 519.809497

8022.515667 158.890643 1732.559600 382.175680 256.868673 1468.657967 369.786033 442.746827 234.793320 2089.228933 308.461293 845.166667 3819.145000 403.766837 380.109233 225.072267 853.734200 286.981227 337.060027 616.355187 1367.601067 824.381317 236.855780 866.497120 217.354330 3241.871433 766.806260 540.249933 167.624017 262.197807 947.766567 947.267390 814.701047 1248.825733 2739.809267 1904.353300 1060.547250 242.186030 308.670603 1179.384733 219.223227

VIL2 WNT10B ZFP36 MLL2 HIST1H3J LTBP4 RGS5 PPFIBP1 PIK3R3 CSDA NPFF HYAL2 TNFSF13 TNFSF12 TNFRSF10C CD84 CFLAR PER2 ST13 F2RL3 SOCS3 CCRL2 SEMA5A DOK2 EDNRB RHOB ARRB1 BCL2L2 C3AR1 CDR1 PLK3 EFNB2 STOM FABP3 FASN FCER1G FDXR GNG11 CD83 CD163 RAB3D

947.676283 1331.265033 236.835037 643.276217 674.101280 317.946253 649.874830 282.316330 183.790823 1298.403600 307.457437 125.452583 1432.403633 479.784883 291.383970 204.975915 183.351617 168.172500 209.272247 1170.126867 2690.652367 301.264343 769.224267 1173.422320 198.561800 238.521473 286.632707 375.490163 320.896393 194.300313 423.589023 579.973077 154.412887 2400.627600 199.530493 498.370780 325.842063 1994.153633 259.390217 755.490220 235.386413

2362.904767 3097.572167 498.995933 1566.262267 1823.285067 1332.136267 1399.790000 569.607627 398.483080 2680.117500 627.475080 369.927327 2999.043733 1156.193933 942.519423 766.719870 527.281070 550.680717 631.828180 2733.274400 5625.982300 605.863240 1919.922000 4850.768133 544.059533 946.742083 614.222830 849.642480 961.947483 864.777467 1092.852070 1556.135100 413.171007 5488.600433 591.758863 2332.586067 683.983567 4358.803800 900.300567 2138.600700 481.635993

C14orf169 MICAL-L2 PTCD2 DENND1A C20orf172 C20orf98 GFM1 TRIM46 MAK3 TARSL1 ELL3 RNF39 COL18A1 SLC38A1 SAA2 TXNDC WNT5B OR2J2 ANP32E TMEM49 TMEM14B PLVAP DKFZp761I2123 RAB34 TSPAN10 TATDN1 PLEKHN1 WDR75 TOMM40L C21orf56 C9orf64 TMEM79 PHF6 C6orf125 CARD11 ASPH USP38 HINT2 C7orf13 C14orf151 KLHL22

51基因芯片_基因芯片

1441.139400 2958.647533 1749.844200 7438.117333 967.969100 6332.952233 1047.741067 1258.876450 600.298633 5465.309233 438.021920 578.260747 1170.559700 546.043950 960.142333 591.184640 4080.500667 1726.009500 1391.840933 1785.460800 4908.072733 11875.915000 3971.429267 3100.305700 772.944720 1078.684533 1349.474533 3193.605300 3780.294833 569.860667 1635.826767 3668.814367 734.684740 626.645023 929.762133 635.145667 772.164243 436.611827 3905.559200 492.584247 20222.656000 1546.718400 935.794400

533.173243 1406.220800 664.162220 2436.168667 414.640417 2228.776967 465.761073 543.135043 147.036580 1447.357600 171.174353 181.564410 537.736433 176.022530 405.098920 293.019220 1790.234467 507.075400 439.612620 840.993180 1490.671800 4948.451333 1043.667690 1548.524233 292.560140 315.890373 533.503510 1280.668900 1790.397133 279.643960 425.897310 1093.986643 317.748047 298.245850 281.558567 252.297890 294.742057 205.803520 901.335887 218.379257 9778.366333 720.616877 452.446690

ASAH1 CEBPA CLCN2 CTSH DMBT1 DUSP1 ERG ETV5 IL16 NOTCH4 OVOL1 4-Sep MAP4K2 RGN SLC16A5 SLC16A4 DHRS3 HS6ST1 C9orf61 SYMPK C1orf38 NAPSA GDF15 AQP3 DAPK1 DOCK2 PPARG RPL3L SCD PLA2G4B HP HR AGTRL1 CBX2 MAP3K8 CSF1R CSTA EVI1 FOS HIST1H1A HCLS1 HIP1 HKR3

217.214097 598.641453 139.746817 201.217917 1185.190667 804.827033 528.212930 253.097997 1131.922200 286.244683 455.020840 3477.139967 613.033690 216.899243 1409.515533 201.013010 211.985867 319.570070 685.263720 319.006687 7657.454433 1103.985467 397.806253 885.372280 489.359417 2175.747467 1942.234133 426.321500 365.430130 574.521150 258.671250 777.252967 230.962447 149.991953 341.873467 1037.138387 1154.050567 116.289333 153.565003 551.527057

901.039053 151.358363 1090.228617

485.327553 1342.160800 297.588607 537.417207 2771.602700 2441.824500 1415.080633 814.771967 2426.218733 631.172127 1666.710200 10193.365500 1244.966567 476.593763 3418.662800 887.359733 591.700180 1575.893500 2280.364933 794.748730 24778.802667 2862.896600 966.885080 1833.704200 1416.954333 7177.949067 6884.082167 1417.253633 1132.789700 2551.610733 523.837300 2273.582333 485.959890 472.344860 892.200450 2182.526933 2353.857867 358.784507 585.900043 1133.253700 2195.336133 352.116873 3215.568500

MASTL C21orf119 C21orf124 RECQL BAD CIP29 PNPT1 RBM18 ANKRD13 CNP CDC2 C19orf33 C20orf72 MAL2 AP1S1 CDKN2A FBXO32 WNT7B BCCIP CDKN2C S100A16 HIST1H2BK WFDC3 C9orf30 WFDC2 WFDC2 WFDC2 WFDC2 MRPL39 DCBLD2 WWOX LRPPRC WHSC1 RHOV NUP35 MTG1 MGC15416 CABYR ALKBH8 RP11-484I6.3 BACE2 SMARCA3 TAF15

16989.480333 371.828120 744.164790 815.416490 4063.583633 1466.694433 593.113873 1236.700267 4056.899833 3310.404900 1262.095067 3314.337400 2630.490733 2491.869300 408.980437 3636.684300 900.227150 5064.746133 2741.242667 1594.834633 2308.658033 432.011183 658.429017 763.134653 1287.595900 2108.516233 662.726043 1365.078000 519.441790 938.749780 2049.723200 9347.019333 615.895680 1224.833500 742.804453 2941.185167 28369.777667 3235.225533 2393.387067 848.443387 7135.298833 2413.381967 13975.952667

8418.382667 168.366433 332.651717 249.150070 1776.838400 431.797473 164.603451 421.982153 1323.738550 666.978383 630.435200 1048.999600 879.402590 872.491333 170.683863 1712.980267 407.967627 1970.540233 1189.194133 687.546167 1037.413077 207.223817 241.845787 359.038457 531.217817 1047.047400 260.669333 276.638020 206.553863 453.677503 866.902017 2618.254633 216.692157 353.194523 325.554727 1133.042647 13271.707000 677.469923 886.158753 415.718613 2692.613567 683.572917 5193.236667

HLA-E HSD11B1 INPP5B INSIG1 LAMP1 LMO2 NFIA NKG7 SGK SLCO2A1 TERF2 NR2F1 KLF10 TMPRSS2 MKRN3 CTDSP2 DNAL4 OACT5 MSLN SPRY1 SMAD7 MIPEP MOCS1 MYLK TSPAN31 TBX2 TBX3 GPR109B TRIM22 HRBL DLC1 HLA-DMA NFATC1 PRKCH PTPN13 TACC1 IFI30 NEDD9 SLC34A2 237.317327 TXNIP FBLN1 FBLN1

736.027020 1613.050800 COMTD1 275.302793 707.876067 NIPA1 551.576050 1312.056367 MGAT5B 623.994947 1686.447867 CRAT 331.151120 798.173700 CDH24 397.801333 1305.041900 HYLS1 547.988407 1239.325767 ACBD5 3990.570000 11341.700500 MGST1 4176.144300 11721.458667 MGST1 236.647977 900.108000 DONSON 204.269353 982.096053 DONSON 219.224970 453.206480 EVC2 879.989193 2060.324367 CTSE 381.629467 894.376913 CHMP4C 2369.858667 9252.780000 TARS 328.329757 962.750990 MGC24665 209.292180 490.810333 ADSSL1 251.185767 941.936123 FRMD6 310.075840 630.418583 C18orf19 377.546113 788.029153 FLJ35779 344.586867 715.393847 C8orf38 231.563330 556.242950 B3GALNT2 589.426123 2437.786200 FAM24B 124.301567 287.838997 IL4I1 222.186520 478.055750 PYDC1 201.034570 424.871340 MGC33214 810.074687 1850.713900 DPM3 477.117450 1050.597390 MLX 633.159213 2890.932067 UNC5CL 838.926117 2219.011833 EFCAB4A 821.546400 2508.576133 WDR62 166.942560

424.045537 FLJ35424 229.715043 496.827087 PDZD8 165.275057 362.634670 CLCN3 123.665235 266.963873 GRIK2 2463.052733 5339.359500 SMOX 1559.530667 4990.064367 SMOX 1928.236300 6245.979100 SMOX 280.089233 630.524910

UHMK1 818.527183

SYT12

619.776730 1596.535200 MRPL47 493.649750 1277.631267 ACTL6A 461.783913

1439.341100

FUT8

710.692967 1606.602167 1515.814033 13194.759333 1619.160233 2785.868700 735.930163 9185.051333 1821.234467 797.022900 475.068307 854.084010 1234.960433 38204.788667 1149.437433 1034.467933 3085.344267 339.549503 3295.821733 1363.078500 2109.626267 2108.612167 790.625717 713.649717 342.943923 3539.974767 706.730370 2933.268933 905.405280 1315.018667 605.005327 1965.288533 1338.839133 4163.981767 327.996507 1760.621867 629.099717 1181.703367 1783.938767 630.134867 1032.762660 2396.868667 1427.638767

246.999697 625.383257 552.803233 3733.658800 707.000067 1223.706333 332.681877 2217.737067 472.237813 249.245693 237.021783 371.211583 600.416017 11400.807433 569.207180 402.305213 1115.086800 140.830057 1507.715167 579.524600 932.436753 873.769567 268.546077 176.252737 169.203535 1381.670033 231.681247 1360.260133 332.030563 330.451830 295.662150 912.967433 464.503643 1927.871567 159.336963 871.965247 305.145823 536.539947 432.586733 247.069317 437.271583 958.808867 702.272697

CPLX1 LILRB1 NMU FOSB AKAP13 SLA SSFA2 MARCO INMT LILRB5 ATF7 LILRB3 ZFP36L2 SFTPA2 SLC2A3 ZNF197 BTN3A3 TACC2 C10orf10 BTN3A2 BTN3A1 PKIG UTRN LST1 PXMP4 SLCO2B1 CD300A ACOT7 GRIN1 CD93 DPF3 RERE LMOD1 FBXO2 HAAO TPSD1 MRAS MUTYH SLC7A8 HEY1 LILRA4 KEAP1 GAB2

426.750690 321.178483 830.714667 744.347253 370.154917 320.193010 357.391050 636.168917 560.063673 492.807390 4447.117200 198.706993 409.939673 7302.729533 2197.420833 1416.178033 154.861740 245.266213 319.799357 970.167267 989.885360 430.977810 172.064305 392.188910 3643.057700 256.405580 186.236947 207.748387 410.547387 167.693803 314.413643 211.823097 357.767583 143.565219 2293.449000 199.630137 277.429327 272.318457 332.421400 228.823903 184.663010 150.677920 480.211007

1632.612333 710.492213 1896.532500 2851.864433 809.815800 657.986867 1290.893183 1411.633533 1140.162400 1036.409500 9129.288333 524.365843 1183.385143 14731.033333 4426.894467 3005.421533 336.506613 671.967333 665.484333 2420.505167 2520.794300 1018.678750 538.940073 802.424167 7975.463200 1231.322967 701.422000 846.380267 867.256747 520.298847 824.693880 530.141880 1418.919900 296.099977 5187.149000 479.043287 750.023273 755.273233 1101.124100 521.616783 576.603627 338.772470 1953.726600

TUSC3 GMCL1 DTX3 FBXO27 C10orf83 STT3B HIF1A CMTM1 CMTM1 EXOSC8 WFDC3 FAM101A ASPHD1 UBE2C LOC144501 FAM101B FLT4 TMPRSS4 C9orf48 LARP6 GNPNAT1 HSPA4 GOLT1A EMB AGRN RAB15 PTHLH PTHLH STAC2 LOC134145 SAA1 GPR150 HOMER2 USP33 TJP2 RBBP8 MGC39606 PPIL5 PPIL5 ENSA ZDHHC24 MYCNOS ASPH

560.308860 616.529620 523.656817 4164.566867 3757.647867 2695.279767 1408.133433 728.001777 456.583923 783.714270 878.054023 2175.907867 651.110410 1327.405467 965.858453 601.942887 1828.446567 1001.691520 2234.808467 421.937650 1233.458400 976.591620 538.389550 839.340277 929.874983 1474.585833 498.854480 2517.022267 680.104453 687.406797 1322.776733 740.656877 1224.716233 9131.749667 655.288520 3045.471933 3029.119033 499.697647 1144.477033 2993.295500 545.849933 2000.857400 848.878757

181.638130 260.549480 246.521027 1861.434200 1620.716367 1127.207463 543.391873 353.059593 204.315743 355.763487 378.299140 979.659380 279.707133 494.207247 313.451793 197.159303 679.806813 469.190980 477.291633 179.097850 520.354057 239.110110 267.303193 300.518187 320.557213 615.465477 202.992210 1078.457890 183.442519 341.114743 402.268410 343.191737 573.154637 4296.659567 233.936773 928.324663 1304.547700 249.488877 414.991557 1291.759300 267.781450 916.674147 392.273203

MYO1F TSPAN12 SLC17A5 C11orf9 SNX11 PKN3 PCOLCE2 PILRA BAZ2B HSD17B8 LIMD1 MAPRE2 ADAMTS7 GCAT NPTXR PIK3R5 VSIG2 IGSF4 APOL3 RAB26 HSPB8 BBC3 CIDEB SIGLEC9 RBMS3 hCAP-H2 GALNT5 LMCD1 SLC40A1 LKAP KIAA0040 KIAA0513 RASSF2 DHCR24 KIAA0672 KIAA0703 MLXIP ZHX2 ABLIM3 NULP1 WDFY3 GGA2 RGL1

597.821447 513.626977 594.888047 699.165867 253.393803 191.596673 347.460997 2070.823067 1954.116200 264.716780 1027.257587 1332.441100 606.552650

165.067410 322.880957 1200.098033 503.023783 1930.285133 104.559930 153.477417 690.822880 456.999070 557.484040 347.219230 143.488757 320.620107 160.521020 197.843907 693.203690 287.123447 202.627833 297.830287 246.703770 623.026700 4204.167000 258.279253 181.864823 392.217480 147.750951 1531.203347 1791.232567 873.830347 681.613523

1346.678833 1347.503467 1279.338800 1477.096000 525.295347 781.137623 812.328640 4923.596567 4844.494633 740.011767 2958.842033 4325.327567 1736.497100 361.614607 1204.699367 3230.251800 1146.693067 4130.054067 221.199003 342.033630 1577.607400 1238.786500 1126.077833 792.286770 453.918307 1242.960200 326.966540 439.890380 2292.919267 643.995057 573.212087 772.981113 595.261273 1481.657100 8577.088667 737.998687 412.053833 912.795313 338.658757 4350.842800 3716.229867 2074.683767 1625.401100

RAGE GPR89A NFKBIE INSR CLTCL1 ZRF1 MICAL3 LOC132969 HSP90AA2 FBXO45 LOC402617 C14orf78 LOC388259 LOC653663 PVT1 C9orf119 LOC391553 LOC391817 LOC399940 LOC400099 LOC401115 LOC401321 LOC440957 LOC441795 FLJ13195 LOC440731 LOC440995 LOC641697 FAM7A2 LOC653191 LOC653235 LOC643240 LOC643664 LOC643988 LOC644151 LOC644294 LOC644447 LOC644586 LOC653505 LOC644733 KRTAP2-1 LOC645139 LOC653583

405.232503 807.325883 4382.187233 1764.424367 2131.381733 729.378147 2053.939867 955.967800 1279.388867 6465.731667 757.200400 478.094940 766.648633 4829.478867 3603.578967 544.059847 1136.013580 4320.099800 494.579187 433.271910 2243.253233 1246.689033 2108.837200 3163.214667 633.714477 13134.034000 1354.780133 1045.328133 5741.436667 913.282917 739.430600 3355.832900 968.634767 720.006723 6752.280300 7272.823300 458.024883 516.132843 2256.199167 556.098243 898.051767 2174.331167 1689.442733

200.338633 369.917003 1577.455500 527.098563 971.895067 332.531647 758.191110 363.191643 390.086103 2915.603333 265.886290 170.387077 360.842683 1978.807233 1337.570400 236.510543 363.422823 1940.250667 137.085692 156.518290 1083.779633 562.789847 422.775390 1454.362400 263.887693 3912.168467 609.305873 295.321697 2813.912800 207.453347 186.067083 1318.916700 459.451273 353.725007 2239.778500 2921.934867 194.696057 247.412507 1087.775067 225.871470 293.880340 788.989967 462.434840

SYNE2 KIAA1026 GPR116 ANKS1A NEDD4L SASH1 SYNE1 DOCK9 TENC1 UNC84B 187.858923 C2orf32 SAMHD1 ZNF385 DST SGEF CCDC69 NOC2L TMEM28 DENND2A WWC3 PPARG STMN3 FAM26B CRYL1 C1QA PLLP KLF13 EIF3S6IP TRPV2 SUFU EGFL7 HECA FZR1 KLF2 TRAP1 BIN2 PLCE1 ABI3 HIGD1B PCDH12 SLC15A3 GYPC

866.084447 1957.352800 LOC645243 318.014793 1549.507967 LOC653594 460.394000 1345.446820 WDR43 164.074687 574.647167 LOC653353 236.228237 504.427273 LOC646256 729.029887 3176.067533 LOC646279 156.763320 420.176857 LOC653741 129.152103 401.169280 LOC646457 203.320417 711.002367 LOC646473 261.996473 1136.577983 PCP4L1 423.919127 LOC646563

568.986537 1303.159200 LOC653397 207.014220 538.734020 LOC646676 558.369757 1262.802900 LOC646993 2036.838067 5033.330500 HSP90AA2 396.105530 859.654100 LOC647250 2073.294400 4762.235267 LOC653524 845.499837 2641.416500 LOC647315 254.156350 583.884907 KIAA0565 431.511647

885.405903 MGC102966 1419.474933 3055.796967 SUZ12P 406.546103 857.964347 LOC646240 254.823920 778.264817 LOC646669 343.621887 1292.432967 LOC653820 154.483943 386.504307 LOC642267 939.406880 2234.252867 LOC441155 614.222430 2057.449833 FAM7A2 755.223233 1615.212667 FAM7A2 660.605167 2049.951400 FAM7A2 140.163383 445.606840 FAM7A2 168.077853 553.276543 FAM7A2 141.451883 333.655273 LOC642759 139.302047 302.036703 MICAL3 439.931580 1019.873867 LOC642974 187.732053 555.474783 LOC642991 852.314353 1953.672900 LOC643278 187.832790 462.539603 LOC643305 455.872947 1017.976730 LOC643354 1453.019200 3069.231833 LOC643253 229.745863 501.198823 LOC643497 243.639167 591.525567 LOC643524 123.539727 266.236913 LOC643665 864.564300

1909.421200

LOC643809

51基因芯片_基因芯片

1418.807967 3961.190233 1320.476733 6350.348300 756.770030 319.140420 1068.638300 741.249567 298.184473 621.674120 1525.815467 2003.037133 411.896560 855.820433 382.498260 286.164750 881.268633 2717.605533 1649.152233 575.868710 545.623773 431.887917 940.596380 2123.732800 3522.660900 1501.319167 3044.049233 419.393293 1149.688633 3913.155300 355.512847 484.419870 512.750317 423.208170 3364.174967 356.036110 25546.068333 22712.624667 2707.448300 4912.132467 710.030950 4022.582433

3527.945867 511.755033 1589.024033 558.891317 2862.969967 337.139873 154.379977 519.063140 341.498757 137.383193 264.212203 662.060293 487.804717 176.616200 397.128337 163.792090 128.476658 251.786487 616.531220 723.905100 217.621427 220.558130 206.122730 234.551747 1042.535800 1717.773800 680.037200 850.310380 182.851517 276.850073 1486.775000 175.660100 165.431932 137.331100 146.729197 896.017377 155.060438 8803.292167 11000.209833 1040.261887 2195.771233 177.111007 1948.860733 962.661833 CECR1 PARP14 KLHDC4 MKNK2 NOTCH1 AHI1 TMEM104 PNRC2 MTMR10 TTC19 WDR8 NALP2 SEMA4G MOSC2 TPCN1 DENND4C FLJ20701 LIMS2 FAM82C PLEKHG6 TMEM140 ACOXL GIMAP4 LMBRD1 FLJ11259 FLJ11292 GIMAP5 CHDH TBX4 TREM1 DEFB103A THSD1 KIAA1166 KIAA1414 575.915557 HES2 HLA-DRA S100A4 C8orf4 STARD7 SEMA3G XAB2 ARRB1 149.745987 391.358257 KIAA0841 222.256727 500.829007 LOC644565 639.673820 1685.971567 LOC644577 1937.821700 4637.236433 C6orf160 293.662990 1166.664733 LOC644769 178.493657 485.136280 LOC644805 206.242263 482.314873 CDKN2C 225.408423 655.561353 LOC645380 517.610207 1564.157200 WDR43 116.121803 244.298550 LOC645687 358.541327 764.293033 LOC644019 1055.525213 2515.657700 LOC644019 2595.024400 5580.108067 LOC644019 1208.759500 3092.535067 LOC644019 1106.160267 2561.992900 LOC644019 467.971080 979.130457 LOC644019 389.931290 910.060013 LOC644019 329.551167 686.835997 LOC644019 170.077273 436.330233 LOC645848 213.500063 563.278550 LOC645941 245.889277 664.780123 PVT1 342.151650 1048.075047 PVT1 321.881163 1121.080453 PVT1 1014.799933 2306.613633 LOC646051 191.621843 706.734533 LOC646201 716.572350 1854.466233 CA5BL 220.671230 865.180887 LOC646720 253.328747 769.765720 LOC646726 401.442130 1304.330290 LOC644663 561.570543 1192.582967 NBPF9 457.105013 1203.131867 LOC646869 161.498997 395.341920 LOC645083 251.627420 642.280050 LOC440421 1240.150933 2896.105733 SUZ12P 1219.610533 NBPF1 1442.481667 2917.544500 LOC653673 1127.798333 3563.585200 LSP1 955.955947 2340.112633 MSH6

391.337250 4587.961100 3502.841367 1530.490533 516.283613 621.826250 440.421200 5265.302633 368.538457 890.945433 1520.165567 1226.946633 589.103380 623.142083 706.391760 2376.870867 399.780163 666.960613 621.337347 1380.211100 816.706833 1481.854833 1876.334633 9490.982667 1108.878580 1180.491533 9370.897333 845.240920 3702.963767 2305.599833 822.518733 4430.780167 2064.993667 2690.432833 590.899567 2057.938933 604.646710 577.408610 1242.723867 438.571023 5205.452133

1171.234940 128.717017 2139.718933 1570.222967 634.850347 148.957453 247.394133 185.088053 2489.563400 134.050705 248.312407 481.019940 454.924317 150.530250 163.120200 298.741740 1181.651543 152.391711 315.785630 286.312663 499.404177 275.978533 601.808667 531.507547 2348.981667 522.731760 535.292367 4222.773900 352.732013 1473.477500 984.265900 274.230620 1421.785500 734.303480 612.460757 264.296390 963.744950 278.701513 288.535490 358.107797 133.279405 2570.876867 490.175440 MAN1C1 SLC44A2 SEPN1 NDRG4 RHOJ ZNF608 CALCOCO1 EPB41L5 CKMT1B HPR CCL3L1 MAGEA5 PYY2 CRY2 MRPL23 CYP4F11 PLEKHB1 FAM60A MID1IP1 LAIR1 ITM2B FXYD6 CXCL16 LHPP PARVG TMBIM1 CARD15 C9orf19 MS4A6A PDE4DIP HIF3A CENTD3 TNS1 SMAP1L TNS3 IPPK RNF38 NDRG4 FGFR2 SIGLEC1 FGFR1

2550.459067 640.547293 611.166907 656.872070 2931.772700 5994.543333 2108.331133 642.046563 2714.060733 1060.814943 685.128547 1839.577467 331.880913 2064.523200 1064.669933 822.750917 414.815410 813.785467 829.071847 853.296250 2323.179733 3118.420000 1143.282300 1465.087710 4452.047533 380.085483 762.272673 424.256960 1313.215233 9425.909333 504.147373 1068.425233 1573.710900 594.358077 482.664137 337.787313 487.433757 652.422327 846.091580 650.362510 1092.913233 851.365220

1533.482000 1162.679367 235.528010 292.176757 294.294540 717.172880 1767.653633 1003.206070 217.806853 1281.030533 338.996443 231.667897 551.226233 151.151390 449.632293 317.618980 369.303837 148.241733 278.496433 308.940773 253.209498 630.140777 1412.108200 355.291800 445.672027 1872.303067 150.738907 350.344430 186.024587 596.861093 4199.215000 197.994738 456.858190 771.623167 178.209357 237.735803 142.302003 177.474940 155.269445 355.835287 295.108040 516.259467 356.638017 758.988037 MS4A4A GDAP1L1 TMEM38A LILRA6 CAPN3 CHPF ADIPOR2 C5orf23 HMBOX1 C16orf30 C1orf115 MMRN2 RNF122 FLJ22662 CYBRD1 PHF17 C6orf59 C14orf159 FRAS1 SOST UPK3B APOL4 WNT3 TCF7L2 EDG8 COLEC12 CD1E UNC93B1 SPRY4 KRTAP1-3 RASSF5 SESN2 ESPN RSHL2 USHBP1 NCALD SLA2 ZMYND15 CAMKK1 HVCN1 ABLIM2 LAT2

403.499613 427.643023 725.798333 1793.276367 3045.537667 2923.685867 531.765553 1550.670400 743.169213 664.117613 769.277243 771.804567 2670.565900 2039.677600 750.522347 1056.265183 885.889353 1096.882867 641.785683 1762.533733 628.297383 424.358473 3078.090833 6642.107167 943.456307 1177.154133 712.300600 8602.457133 764.118347 480.099313 793.400167 980.294633 6581.073600 1221.657267 819.473777 1089.354447 674.324393 1303.562300 12812.174667 10808.565333 9559.654667

785.779643 191.419727 204.068100 315.739713 716.661833 1315.912633 1334.544933 189.769078 620.250313 302.680460 326.837287 226.847977 202.029992 1060.950067 803.872463 271.924003 334.588370 295.229130 360.945530 220.175703 778.148553 257.539157 179.791990 750.412967 3240.410167 391.687910 512.134700 285.058257 1731.917333 314.531913 148.401797 236.823599 300.964700 2879.664633 383.618243 332.844437 507.134690 271.754307 462.273977 4420.266967 3213.748533 3252.968767 345.753917 DAB2IP EMR3 PHACS COX4I2 GATA2 GPR124 MFSD2 C1orf198 C3orf39 LRP11 TNS4 CGNL1 CCL14 SYNE1 NEK9 ADAM19 CASP1 CASP1 CASP1 SSH2 GZMH C9orf28 SLC38A5 HLA-DPA1 RCSD1 MGC17330 COP1 HSPA12B OLFM2 ABCA10 4-Sep 4-Sep PTPN6 TBX5 COLEC12 KLHL6 OSCAR TSPAN18 DCN DCN DCN DTX3L

350.800560 3010.843367 5161.208833 349.497003 1193.536067 1903.192367 2345.212667 1396.574367 801.503570 2305.834267 1691.166367 4407.763467 467.163200 2177.108067 507.236637 572.368900 1423.027267 775.630063 549.894890 1148.608300 303.427597 331.242600 1304.909433 1230.737533 428.294793 717.578893 1153.664267 5056.605000 600.032453 489.874503 3160.410700 677.653750 489.564553 823.863850 1049.983667 662.356173 1672.152767 1577.318367 571.873453 1465.977367

938.889767 161.734840 1072.848400 2501.530067 151.895697 536.975623 816.712583 1028.044667 305.117833 227.766803 838.411950 791.308777 2119.624967 183.105637 904.921743 243.663067 225.695740 616.608453 156.498623 227.219980 542.871540 131.746797 152.858797 363.472007 432.930303 161.236443 334.862660 513.801517 1286.463353 271.317297 146.613563 790.234667 241.085887 232.827547 297.712000 439.812797 284.617187 380.344207 612.998643 216.937560 481.354073 277.061790 LOC91461 REEP6 CMTM7 C10orf65 RHCE TRIOBP ESAM CD300LF NIP TMEM125 PPM1M IGJ CMTM2 RP11-5G9.1 SUSD3 NEIL2 MGC7036 CHRDL1 C17orf50 ATPAF2 NCR3 RG9MTD2 GYLTL1B TNFAIP8L1 SLC44A3 NUDT16 FAM69B MGC45438 C17orf77 RNF165 FGD5 C9orf66 SAMD9L FLJ30313 MS4A6A APCDD1 AMICA1 GIMAP7 MGC45491 MGC33486 TMEM139

51基因芯片_基因芯片

608.493963 869.391140 873.597733 994.462737 965.764447 1023.280500 994.173233 8942.192833 2323.840300 350.999303 988.235213 490.501313 2362.160267 4253.601067 2988.612167 721.634713 405.091800 434.764123 2639.182500 624.760033 3315.898067 2085.210100 609.911520 2215.872033 3405.094067 1665.755100 410.389690 3437.559933 666.553413 537.658923 2035.513267 4015.362700 494.835560 527.525350 5619.692100 2773.851733 352.190157 735.602967 3131.148000 3190.353933

835.185643 256.875760 193.055157 327.450793 388.257907 195.759720 274.943640 261.575575 3745.735567 803.569900 119.416263 227.996177 207.725950 501.173437 951.312413 1471.579100 339.431377 172.714637 198.177313 1273.123933 254.002810 1081.396333 497.804693 192.637677 1044.249083 1417.926800 672.549233 203.851840 1481.113300 275.953000 204.061083 999.281167 1794.402333 184.639130 211.587037 2441.051133 1081.167667 166.522017 341.555567 1195.352067 1011.365557 242.760917 PLAC2 CSAG1 CPNE7 CSF3R CSF3R SH2D3C KCNMB3 TNFSF13 TFAP2D CSF3R NFATC1 CAPN3 NR4A1 TGIF RNF152 FGD2 SLC9A9 FLJ34047 ARHGEF15 C1orf162 CLEC14A STX12 SULT1A2 SULT1A1 SULT1A1 TREML1 PILRA RTN4RL1 LOC201164 RNF166 ID1 PIK3R1 GRASP PKIG BCL6B RXFP4 RASSF5 UPK3B UPK3B NEDD9

512.685000 1469.063400 1824.745233 1261.478933 589.932250 35487.681000 1502.889500 986.693400 648.942760 554.906303 757.027953 666.265333 616.667890 862.160700 6472.150000 1755.658467 2145.124833 743.416423 935.538567 2535.964600 759.299210 446.372040 1295.953267 730.100893 485.962583 874.522200 2296.357267 350.844920 3580.006767 659.749117 569.451923 2127.956267 2085.851533 404.840487 1236.191057 1068.171217 777.737360 1147.566377 473.153730 2561.939700 3865.181500 3247.811367

852.908810 210.316577 587.106543 869.796587 583.568467 164.134090 14735.949667 731.401347 406.526883 229.570400 184.509063 250.808977 198.322348 176.974997 363.897053 2846.989067 686.958583 731.328320 302.925890 361.179830 1235.849433 284.049623 195.305525 631.931467 361.854557 194.251063 263.010893 648.760287 163.015000 1144.996457 329.564687 140.687022 722.040247 558.231647 163.223883 448.030983 511.326100 383.982390 500.246757 136.835767 813.479180 1765.154133 749.839200 407.336590 TRIM55 RNASE4 RNASE4 BTN3A1 ANKRD47 SFTPB MKNK1 CXCL12 TCL1B SPRY1 THRA CLEC12A CLEC12A TCF7 MGC35402 TMEM88 CD163 IER5L DOCK8 SFTPG OSCAR UNQ5783 MYADML FLJ42953 FLJ41841 PLA2G4F PTAFR DARC NR4A3 N/A PDE4A NFIX ITPR1 BMPR2 N/A GAB1 DVL1 CHIT1 NOTCH4 ASS VWF RARA

1308.671767 465.512257 4871.203433 407.047330 1508.064233 509.657533 571.237640 1445.278180 571.481010 9587.210800 379.611007 1152.421990 1169.495767 1475.410633 1439.939700 1286.048400 2685.854567 919.297043 456.564807 2359.466433 353.194487 585.797780 1715.186367 3768.945567 3899.861000 852.808080 2411.319500 437.990940 1735.888767

1681.725933 382.841623 186.382197 1481.548800 202.089263 624.123833 236.382783 173.600293 395.617200 208.946747 3935.603533 140.337290 545.485443 284.180297 375.179287 552.208237 561.406747 1272.275400 377.640353 199.304043 1011.190683 172.106453 261.638073 611.896267 1296.843600 1177.236600 374.288093 1130.067600 188.330997 750.339783 718.055947 N/A GATA6 SHANK3 MAST3 BEXL1 LBA1 KIF26A KIAA1922 FLJ44379 LOC124685 LOC132706 LOC138652 HEG1 KIAA1462 DTX4 SIRPB2 tcag7.216 LOC285479 ARHGAP23 LRFN1 LOC343384 LOC340947 LOC341112 LOC341356 LOC347454 LOC340096 LOC653643 LOC388210 PEAR1

9513.320333 1370.650067 385.400360 3879.018333 1392.039967 1114.168833 6331.053967 410.198410 8733.746000 1078.608747 358.810963 1204.432067 4247.248633 1369.076133 9999.704000 476.326587 793.196463 5640.080700 761.776867 490.395953 441.782627 344.722487

3307.505100 4651.268000 458.984417 160.394343 1147.067800 451.582740 504.824807 2710.514100 168.586637 3022.282067 438.381237 147.351243 591.670590 1985.816600 612.972030 4349.862000 209.759520 233.220830 1654.200933 327.449403 209.452950 177.873940 163.094790 1382.303000 LOC390998 LOC391045 LOC391269 LOC391359 LOC391555 LOC391742 LOC392179 GOLGA8C LOC402716 LOC400019 LOC401093 LOC401284 LOC440359 FLJ35282 LOC389787 LOC441511 LOC338586 LOC390364 LOC127406 LOC442251 LOC442329 LOC442424 LOC64501

1607.140167 463.529973 245.659847 3023.572200 349.862060 488.823420 373.905620 230.913887 1767.511533 1017.813680 1391.465500 836.018967 10497.009167 24934.916667 4243.566067 891.938077 353.438740 7222.742233 1868.429333 732.652663 3540.142067

639.662813 571.674910 181.192153 117.893763 1493.868867 136.774027 200.125903 166.720263 109.598105 743.835613 269.440693 473.608163 404.182470 4099.376167 8067.738000 2067.845633 334.876953 173.575410 2998.987867 403.986323 216.614687 1756.687633 297.910867 3 LOC441378 LOC642408 LOC642447 LOC642480 LOC653086 LOC642646 LOC642772 LOC643261 LOC643302 LOC643425 LOC653253 LOC643448 LOC643563 LOC392506 LOC340598 LOC643699 LOC441609 LOC643897 LOC643900 AATK LOC644227 LOC644265

51基因芯片_基因芯片

2130.734400

634.395467

2453.689400

864.416280

373.829200

3946.178000

1508.330400

455.557950

351.874257

2919.679033

596.669600

1851.348633

6311.105600

958.571657

598.775673

762.244410

1080.393250

1273.803067

1376.055867

631.042630

578.979530

3221.597333

2007.719200 494.656787 262.217847 910.666833 388.491627 155.455075 1722.515333 649.382080 219.892423 163.734650 1027.374900 218.164360 718.672947 2912.070400 447.709147 241.525797 282.199933 364.143533 594.569787 681.497047 293.743037 258.409113 1302.232720 954.812173 LOC644310 LOC619207 LOC644436 PRDM6 LOC644540 LOC644584 LOC440104 LOC391799 LOC644666 LOC644711 LOC644785 LOC653840 LOC644862 LOC644863 LOC644897 LOC644944 LOC653568 LOC645179 LOC643787 LOC645349 LOC645397 LOC645452 LOC64546

382.579037

623.992077

798.578450

435.083463

434.027613

1518.322300

688.492367

5135.250667

520.933183

3442.259100

1750.093133

8558.086000

1450.314167

702.543387

501.956370

2271.591700

901.315020

1096.993600

3506.837267

2479.208100

1929.066200

670.987377 131.786210 281.993537 363.213030 192.528833 140.151830 757.758707 274.590093 2419.086500 245.258243 1093.096300 523.108320 4125.767733 565.673903 281.897990 245.186277 966.434667 348.580080 415.619223 876.814493 1031.937217 646.149553 250.145763 3 LOC645545 LOC645623 LOC645626 LOC645659 ESPNP LOC653361 LOC646082 LOC646119 LOC653711 LOC646163 LOC646428 LOC646433 LOC653747 LOC646521 LOC646529 LOC644739 LOC653764 LOC646662 LOC646781 LOC647066 LOC647082 LOC647130

815.540827

2335.560267

810.352013

43164.968667

574.324927

740.941023

1275.664300

731.675333

1448.131700

1650.710400

811.398457

590.768680

603.854447

2179.696100

6932.675900

6393.368667

989.606553

620.616150

755.157867

578.211450

748.238117

681.028770

817.216763

1376.702133

746.142067 217.929193 1158.742853 375.037793 14016.127333 158.281868 296.238587 610.394267 292.287700 597.523353 789.050530 348.286420 193.107140 294.124983 1073.946167 3424.879233 3188.964833 256.692943 151.410080 307.453990 164.985133 216.491700 217.223370 347.744037 644.941713 267.397470 LOC644207 LOC647165 LOC647195 LOC653509 LOC647272 LOC647289 LOC647295 LOC653698 LOC653562 ID2B FLJ43663 LOC641700 FLJ31033 LOC653136 LOC284821 LOC284821 C10orf128 FAM92A1 LOC283683 LOC653107 LOC653107 LOC653107 LOC653107 LOC642778 LOC64350

692.412133

1261.803767

361.368967

665.633330

353.145057

1422.835333

1315.266900

1635.966200

4079.760400

309.564380

427.747770

309.603780

2096.762867

2525.480667

1152.859233

684.106653

2004.329467

543.001167

319.251523

626.165247

1263.443633

344.743040

446.279753

1145.034033

310.833880 231.460620 499.597220 135.131290 193.540100 164.789823 675.406000 373.216330 611.615410 1920.457067 116.838238 193.188090 135.666448 896.164650 1194.371700 531.190843 315.801170 566.143640 250.695477 129.663753 157.418510 595.402417 147.297290 130.000870 537.503253 140.474270 4 ANXA8L1 DDX12 PLEKHA2 LOC644477 LOC644695 LOC653840 LOC653840 LOC653840 LOC644992 LOC653584 LOC653275 LOC645717 FLJ41352 FLJ41352 LOC653361 LOC653361 LOC653361 LOC646229 C20orf74 LOC643669 LOC646566 LOC646571 LOC646606 JMJD3 LOC653787

358.700510

491.176853

1400.336943

944.130610

26323.610333

37545.552333

1917.104600

5297.537600

410.883437

2358.431767

1426.700967

5456.289300

645.829053

731.836387

795.053847

469.310653

896.091850

1246.375567

2349.705567 173.773870 215.741180 669.993947 362.208263 6648.666333 12700.528000 512.036773 2290.213100 196.607660 985.065893 592.448860 2395.019367 304.707583 346.354187 386.527260 214.976263 294.514823 582.054790 783.323083 NBPF9 MGC5139 LOC653539 LOC647243 LOC653509 LOC653509 LOC653562 LOC653562 XRRA1 LOC392843 LOC653720 ID2B FLJ43663 LOC654350 LOC654347 PIK3C2B LST1 PRKCZ CD163

四 : 基因芯片的主要类型

基因芯片 -主要类型基因芯片 基因芯片的主要类型基因芯片检测目前已有多种方法可以将寡核苷酸或短肽固定到固相支持物上。这些方法总体上有两种，即原位合成（ in situ synthesis ）与合成点样两种。支持物有多种如玻璃片、硅片、聚丙烯膜、硝酸纤维素膜、尼龙膜等，但需经特殊处理。作原位合成的支持物在聚合反应前要先使其表面衍生出羟基或氨基（视所要固定的分子为核酸或寡肽而定）并与保护基建立共价连接；作点样用的支持物为使其表面带上正电荷以吸附带负电荷的探针分子，通常需包被以氨基硅烷或多聚赖氨酸等。

原位合成法主要为光引导聚合技术（ Light-directed synthesis ），它不仅可用于寡聚核苷酸的合成，也可用于合成寡肽分子。光引导聚合技术是照相平板印刷技术（ photolithography ）与传统的核酸、多肽固相合成技术相结合的产物。半导体技术中曾使用照相平板技术法在半导体硅片上制作微型电子线路。固相合成技术是当前多肽、核酸人工合成中普遍使用的方法，技术成熟且已实现自动化。二者的结合为合成高密度核酸探针及短肽阵列提供了一条快捷的途径。

以合成寡核苷酸探针为例，该技术主要步骤为：首先使支持物羟基化，并用光敏保护基团将其保护起来。每次选取择适当的蔽光膜（ mask ）使需要聚合的部位透光，其它部们不透光。这样，光通过蔽光膜照射到支持物上，受光部位的羟基解保护。因为合成所用的单体分子一端按传统固相合成方法活化，另一端受光敏保护基的保护，所以发生偶联的部位反应后仍旧带有光敏保护基团。因此，每次通过控制蔽光膜的图案（透光与不透光）决定哪些区域应被活化，以及所用单体的种类和反应次序就可以实现在待定位点合成大量预定序列寡聚体的目的。

该方法的主要优点是可以用很少的步骤合成极其大量的探针阵列。例如，合成 48 （ 65536 ） 个探针的 8 聚体寡核苷酸序列仅需 4 × 8=32 步操作， 8 小时就可以完成。而如果用传统方法合成然后点样，那么工作量的巨大将是不可思议的。同时，用该方法合成的探针阵列密度可高达到 106/cm2 。不过，尽管该方法看来比较简单，实际上并非如此。主要原因是，合成反应每步产率比较低，不到 95% 。而通常固相合成反应每步的产率在 99% 以上。因此，探针的长度受到了限制。而且由于每步去保护不很彻底，致使杂交信号比较模糊，信噪比降低。为此有人将光引导合成技术与半异体工业所用的光敏抗蚀技术相结合，以酸作为去保护剂，使每步产率增加到 98% 。原因是光敏抗蚀剂的解离对照度的依赖是非线性的，当照度达到特定的阈值以上保护剂就会解离。所以，该方法同时也解决了由于蔽光膜透光孔间距离缩小而引起的光衍射问题，有效地提高了聚合点阵的密度。另据报导 ，利用波长更短的物质波如电子射线去除保护可使点阵密度达到 1010/cm2 。

除了光引导原位合成技术外，有的公司如美国 Incyte Pharmaceuticals 等使用压电打印法 (Piezoelectric printing) 进行原位合成。其装置与普通的彩色喷墨打印机并无两样，所用技术也是常规的固相合成方法。做法是将墨盒中的墨汁分别用四种碱基合成试剂所替代，支持物经过包被后，通过计算机控制喷墨打印机将特定种类的试剂喷洒到预定的区域上。冲洗、去保护、偶联等则同于一般的固相原位合成技术。如此类推，可以合成出长度为 40 到 50 个碱基的探针，每步产率也较前述方法为高，可达到 99% 以上。

尽管如此，通常原位合成方法仍然比较复杂，除了在基因芯片研究方面享有盛誉的 Affymetrix 等公司使用该技术合成探针外，其它中小型公司大多使用合成点样法。

后一方法在多聚物的设计方面与前者相似，合成工作用传统的 DNA 或多肽固相合成仪完成，只是合成后用特殊的自动化微量点样装置将其以比较高的密度涂布于硝酸纤维膜、尼龙膜或玻片上。支持物应事先进行特定处理，例如包被以带正电荷的多聚赖酸或氨基硅烷。现在已有比较成型的点样装置出售，如美国 Biodot 公司的点膜产品以及 Cartesian Technologies 公司的 PixSys NQ/PA 系列产品。前者产生的点阵密度可以达到 400/cm2 ，后者则可达到 2500/cm2 。

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